Abstract
The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within V. anguillarum (designated O1 to O16) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different serotypes for the first three serovars: O1, O2, and O3. Moreover, immunoblotting showed that strains belonging to each of these serotypes had the same LPS banding pattern independent of the origin of the strain. Serotype O2 contains different subtypes, O2a and O2b. While no differences were apparent between these subgroups in silver-stained gels, they could be separated by quantitative agglutination (titer determination) or immunoblotting. V. ordalii, the former biotype II of V. anguillarum, strongly reacts with anti-V. anguillarum O2a antiserum. Strains of the two species can be separated on the basis of different LPS profiles in the high-molecular-weight region of silver-stained gels of purified LPS. The silver-stained LPS profiles of the different serotypes of V. anguillarum that have been established are provided for further comparison in the future.
Full Text
The Full Text of this article is available as a PDF (509.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
- Chart H., Trust T. J. Characterization of the surface antigens of the marine fish pathogens Vibrio anguillarum and Vibrio ordalii. Can J Microbiol. 1984 May;30(5):703–710. doi: 10.1139/m84-105. [DOI] [PubMed] [Google Scholar]
- Cisar J. O., Fryer J. L. An epizootic of vibriosis in chinook salmon. Wildl Dis. 1969 Apr;5(2):73–76. doi: 10.7589/0090-3558-5.2.73. [DOI] [PubMed] [Google Scholar]
- Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mutharia L. W., Raymond B. T., Dekievit T. R., Stevenson R. M. Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and serotype 0:2 strains of Vibrio anguillarum. Can J Microbiol. 1993 May;39(5):492–499. doi: 10.1139/m93-070. [DOI] [PubMed] [Google Scholar]
- Pacha R. E., Kiehn E. D. Characterization and relatedness of marine vibrios pathogenic to fish: physiology, serology, and epidemiology. J Bacteriol. 1969 Dec;100(3):1242–1247. doi: 10.1128/jb.100.3.1242-1247.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sørensen U. B., Larsen J. L. Serotyping of Vibrio anguillarum. Appl Environ Microbiol. 1986 Mar;51(3):593–597. doi: 10.1128/aem.51.3.593-597.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tubiash H. S., Colwell R. R., Sakazaki R. Marine vibrios associated with bacillary necrosis, a disease of larval and juvenile bivalve mollusks. J Bacteriol. 1970 Jul;103(1):271–272. doi: 10.1128/jb.103.1.271-272.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]