Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 Feb;62(2):429–436. doi: 10.1128/aem.62.2.429-436.1996

Growth Rate-Dependent Modulation of Carbon Flux through Central Metabolism and the Kinetic Consequences for Glucose-Limited Chemostat Cultures of Corynebacterium glutamicum

M Cocaign-Bousquet, A Guyonvarch, N D Lindley
PMCID: PMC1388769  PMID: 16535231

Abstract

The physiological behavior of Corynebacterium glutamicum in glucose-limited chemostat cultures was examined from both growth kinetics and enzymatic viewpoints. Metabolic fluxes within the central metabolism were calculated from growth kinetics and analyzed in relation to specific enzyme activities. At high growth rates, incomplete glucose removal was observed, and this was attributed to rate-limiting capacity of the phosphotransferase system transporter and the probable contribution of a low-affinity permease uptake mechanism. The improved biomass yield observed at high growth rates was related to a shift in the profile of anaplerotic carboxylation reactions, with pyruvate carboxylase replacing malic enzyme. Phosphoenolpyruvate carboxylase, an activity often assumed to be the major anaplerotic reaction during growth of C. glutamicum on glucose, was present at only low levels and is unlikely to contribute significantly to tricarboxylic acid cycle fuelling other than at low growth rates.

Full Text

The Full Text of this article is available as a PDF (253.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON N. G. Analytical techniques for cell fractions. II. A spectrophotometric column monitoring system. Anal Biochem. 1962 Oct;4:269–283. doi: 10.1016/0003-2697(62)90088-x. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang J. Y., Knecht R., Braun D. G. Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides. Biochem J. 1981 Dec 1;199(3):547–555. doi: 10.1042/bj1990547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dietrich J., Henning U. Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite. Eur J Biochem. 1970 Jun;14(2):258–269. doi: 10.1111/j.1432-1033.1970.tb00285.x. [DOI] [PubMed] [Google Scholar]
  5. Hinman L. M., Blass J. P. An NADH-linked spectrophotometric assay for pyruvate dehydrogenase complex in crude tissue homogenates. J Biol Chem. 1981 Jul 10;256(13):6583–6586. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lin R. I., Schjeide O. A. Micro estimation of RNA by the cupric ion catalyzed orcinol reaction. Anal Biochem. 1969 Mar;27(3):473–483. doi: 10.1016/0003-2697(69)90061-x. [DOI] [PubMed] [Google Scholar]
  8. Mori M., Shiio I. Purification and some properties of phosphoenolpyruvate carboxylase from Brevibacterium flavum and its aspartate-overproducing mutant. J Biochem. 1985 Apr;97(4):1119–1128. doi: 10.1093/oxfordjournals.jbchem.a135156. [DOI] [PubMed] [Google Scholar]
  9. Neijssel O. M., Hardy G. P., Lansbergen J. C., Tempest D. W., O'Brien R. W. Influence of growth environment on the phosphoenolpyruvate: glucose phosphotransferase activities of Escherichia coli and Klebsiella aerogenes: a comparative study. Arch Microbiol. 1980 Mar;125(1-2):175–179. doi: 10.1007/BF00403216. [DOI] [PubMed] [Google Scholar]
  10. O'Brien R. W., Neijssel O. M., Tempest D. W. Glucose phosphoenolpyruvate phosphotransferase activity and glucose uptake rate of Klebsiella aerogenes growing in chemostat culture. J Gen Microbiol. 1980 Feb;116(2):305–314. doi: 10.1099/00221287-116-2-305. [DOI] [PubMed] [Google Scholar]
  11. Ozaki H., Shiio I. Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. II. Regulation of phosphoenolpyruvate carboxylase and pyruvate kinase. J Biochem. 1969 Sep;66(3):297–311. doi: 10.1093/oxfordjournals.jbchem.a129148. [DOI] [PubMed] [Google Scholar]
  12. Rollin C., Morgant V., Guyonvarch A., Guerquin-Kern J. L. 13C-NMR studies of Corynebacterium melassecola metabolic pathways. Eur J Biochem. 1995 Jan 15;227(1-2):488–493. doi: 10.1111/j.1432-1033.1995.tb20414.x. [DOI] [PubMed] [Google Scholar]
  13. Snoep J. L., de Graef M. R., Teixeira de Mattos M. J., Neijssel O. M. Pyruvate catabolism during transient state conditions in chemostat cultures of Enterococcus faecalis NCTC 775: importance of internal pyruvate concentrations and NADH/NAD+ ratios. J Gen Microbiol. 1992 Oct;138(10):2015–2020. doi: 10.1099/00221287-138-10-2015. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES