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. 1996 Mar;62(3):761–765. doi: 10.1128/aem.62.3.761-765.1996

Isolation and Characterization of a Facultatively Aerobic Bacterium That Reductively Dehalogenates Tetrachloroethene to cis-1,2-Dichloroethene

P K Sharma, P L McCarty
PMCID: PMC1388792  PMID: 16535267

Abstract

A rapidly-growing facultatively aerobic bacterium that transforms tetrachloroethene (PCE) via trichloroethene (TCE) to cis-1,2-dichloroethene (cis-1,2-DCE) at high rates in a defined medium was isolated from a contaminated site. Metabolic characterization, cellular fatty acid analysis, and partial sequence analysis of 16S rRNA showed that the new isolate, strain MS-1, has characteristics matching those of the members of the family Enterobacteriaceae. Strain MS-1 can oxidize about 58 substrates including many carbohydrates, short-chain fatty acids, amino acids, purines, and pyrimidines. It can transform up to 1 mM PCE (aqueous) at a rate of about 0.5 (mu)mol of PCE(middot) h(sup-1)(middot)mg (dry weight) of cell(sup-1). PCE transformation occurs following growth on or with the addition of single carbon sources such as glucose, pyruvate, formate, lactate, or acetate or with complex nutrient sources such as yeast extract or a mixture of amino acids. PCE dehalogenation requires the absence of oxygen, nitrate, and high concentrations of fermentable compounds such as glucose. Enterobacter agglomerans biogroup 5 (ATCC 27993), a known facultative bacterium that is closely related to strain MS-1, also reductively dehalogenated PCE to cis-1,2-DCE. To our knowledge, this is the first report on isolation of a facultative bacterium that can reductively transform PCE to cis-1,2-DCE under defined physiological conditions. Also, this is the first report of the ability of E. agglomerans to dehalogenate PCE.

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Selected References

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  1. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
  3. Castro C. E., Wade R. S., Belser N. O. Biodehalogenation: reactions of cytochrome P-450 with polyhalomethanes. Biochemistry. 1985 Jan 1;24(1):204–210. doi: 10.1021/bi00322a029. [DOI] [PubMed] [Google Scholar]
  4. Criddle C. S., DeWitt J. T., Grbić-Galić D., McCarty P. L. Transformation of carbon tetrachloride by Pseudomonas sp. strain KC under denitrification conditions. Appl Environ Microbiol. 1990 Nov;56(11):3240–3246. doi: 10.1128/aem.56.11.3240-3246.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Criddle C. S., DeWitt J. T., McCarty P. L. Reductive dehalogenation of carbon tetrachloride by Escherichia coli K-12. Appl Environ Microbiol. 1990 Nov;56(11):3247–3254. doi: 10.1128/aem.56.11.3247-3254.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiStefano T. D., Gossett J. M., Zinder S. H. Hydrogen as an electron donor for dechlorination of tetrachloroethene by an anaerobic mixed culture. Appl Environ Microbiol. 1992 Nov;58(11):3622–3629. doi: 10.1128/aem.58.11.3622-3629.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Egli C., Tschan T., Scholtz R., Cook A. M., Leisinger T. Transformation of tetrachloromethane to dichloromethane and carbon dioxide by Acetobacterium woodii. Appl Environ Microbiol. 1988 Nov;54(11):2819–2824. doi: 10.1128/aem.54.11.2819-2824.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Enzien M. V., Picardal F., Hazen T. C., Arnold R. G., Fliermans C. B. Reductive Dechlorination of Trichloroethylene and Tetrachloroethylene under Aerobic Conditions in a Sediment Column. Appl Environ Microbiol. 1994 Jun;60(6):2200–2204. doi: 10.1128/aem.60.6.2200-2204.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fathepure B. Z., Nengu J. P., Boyd S. A. Anaerobic bacteria that dechlorinate perchloroethene. Appl Environ Microbiol. 1987 Nov;53(11):2671–2674. doi: 10.1128/aem.53.11.2671-2674.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holliger C., Schraa G., Stams A. J., Zehnder A. J. A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth. Appl Environ Microbiol. 1993 Sep;59(9):2991–2997. doi: 10.1128/aem.59.9.2991-2997.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kästner M. Reductive dechlorination of Tri- and tetrachloroethylenes depends on transition from aerobic to anaerobic conditions. Appl Environ Microbiol. 1991 Jul;57(7):2039–2046. doi: 10.1128/aem.57.7.2039-2046.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Langlois B. E. Reductive dechlorination of DDT by Escherichia coli. J Dairy Sci. 1967 Jul;50(7):1168–1170. doi: 10.3168/jds.S0022-0302(67)87587-8. [DOI] [PubMed] [Google Scholar]
  13. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]

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