Abstract
Four pure cultures of denitrifying bacteria, which had previously been isolated on defined alkylbenzenes, were capable of anaerobic growth with crude oil as the only source of organic substrates. Chemical analyses after growth revealed that the known growth substrates toluene, ethylbenzene, and m-xylene were selectively consumed from the oil. o-Xylene and p-xylene, which as pure compounds did not support growth, were consumed to a lesser extent.
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- Altenschmidt U., Fuchs G. Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate. Arch Microbiol. 1991;156(2):152–158. doi: 10.1007/BF00290990. [DOI] [PubMed] [Google Scholar]
- Dean B. J. Recent findings on the genetic toxicology of benzene, toluene, xylenes and phenols. Mutat Res. 1985 Nov;154(3):153–181. doi: 10.1016/0165-1110(85)90016-8. [DOI] [PubMed] [Google Scholar]
- Dolfing J., Zeyer J., Binder-Eicher P., Schwarzenbach R. P. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol. 1990;154(4):336–341. doi: 10.1007/BF00276528. [DOI] [PubMed] [Google Scholar]
- Edwards E. A., Grbić-Galić D. Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl Environ Microbiol. 1994 Jan;60(1):313–322. doi: 10.1128/aem.60.1.313-322.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards E. A., Grbić-Galić D. Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions. Appl Environ Microbiol. 1992 Aug;58(8):2663–2666. doi: 10.1128/aem.58.8.2663-2666.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards E. A., Wills L. E., Reinhard M., Grbić-Galić D. Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. Appl Environ Microbiol. 1992 Mar;58(3):794–800. doi: 10.1128/aem.58.3.794-800.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans P. J., Ling W., Goldschmidt B., Ritter E. R., Young L. Y. Metabolites formed during anaerobic transformation of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization. Appl Environ Microbiol. 1992 Feb;58(2):496–501. doi: 10.1128/aem.58.2.496-501.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans P. J., Mang D. T., Kim K. S., Young L. Y. Anaerobic degradation of toluene by a denitrifying bacterium. Appl Environ Microbiol. 1991 Apr;57(4):1139–1145. doi: 10.1128/aem.57.4.1139-1145.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fries M. R., Zhou J., Chee-Sanford J., Tiedje J. M. Isolation, characterization, and distribution of denitrifying toluene degraders from a variety of habitats. Appl Environ Microbiol. 1994 Aug;60(8):2802–2810. doi: 10.1128/aem.60.8.2802-2810.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Häner A., Höhener P., Zeyer J. Degradation of p-xylene by a denitrifying enrichment culture. Appl Environ Microbiol. 1995 Aug;61(8):3185–3188. doi: 10.1128/aem.61.8.3185-3188.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovley D. R., Coates J. D., Woodward J. C., Phillips E. Benzene oxidation coupled to sulfate reduction. Appl Environ Microbiol. 1995 Mar;61(3):953–958. doi: 10.1128/aem.61.3.953-958.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovley D. R., Lonergan D. J. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15. Appl Environ Microbiol. 1990 Jun;56(6):1858–1864. doi: 10.1128/aem.56.6.1858-1864.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rabus R., Nordhaus R., Ludwig W., Widdel F. Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. Appl Environ Microbiol. 1993 May;59(5):1444–1451. doi: 10.1128/aem.59.5.1444-1451.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rabus R., Widdel F. Anaerobic degradation of ethylbenzene and other aromatic hydrocarbons by new denitrifying bacteria. Arch Microbiol. 1995 Feb;163(2):96–103. doi: 10.1007/BF00381782. [DOI] [PubMed] [Google Scholar]
- Rabus R., Widdel F. Conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain Tol2. Arch Microbiol. 1995 Dec;164(6):448–451. doi: 10.1007/BF02529744. [DOI] [PubMed] [Google Scholar]
- Rueter P., Rabus R., Wilkes H., Aeckersberg F., Rainey F. A., Jannasch H. W., Widdel F. Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria. Nature. 1994 Dec 1;372(6505):455–458. doi: 10.1038/372455a0. [DOI] [PubMed] [Google Scholar]
- Schocher R. J., Seyfried B., Vazquez F., Zeyer J. Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch Microbiol. 1991;157(1):7–12. doi: 10.1007/BF00245327. [DOI] [PubMed] [Google Scholar]
- Seyfried B., Glod G., Schocher R., Tschech A., Zeyer J. Initial reactions in the anaerobic oxidation of toluene and m-xylene by denitrifying bacteria. Appl Environ Microbiol. 1994 Nov;60(11):4047–4052. doi: 10.1128/aem.60.11.4047-4052.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]