Abstract
Cell extracts (high-speed [150,000 x g] supernatants) from Pseudomonas fluorescens NCIMB 11764 catalyzed the oxidation of cyanide to CO(inf2) (and NH(inf3)). Conversion was both oxygen and NADH dependent, with 1 mol of each being consumed per mol of cyanide degraded. Analysis of (sup13)CO(inf2) by mass spectrometry indicated that one atom each of isotopically labelled oxygen 18 from molecular oxygen and water were incorporated during enzymatic conversion. The results confirm earlier reports of oxygenase-mediated cyanide conversion in this organism. A reaction pathway for cyanide oxidation involving initial monooxygenation followed by hydrolysis of a hypothetical oxygenated intermediate to CO(inf2) (and NH(inf3)) is proposed.
Full Text
The Full Text of this article is available as a PDF (240.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Harris R., Knowles C. J. Isolation and growth of a Pseudomonas species that utilizes cyanide as a source of nitrogen. J Gen Microbiol. 1983 Apr;129(4):1005–1011. doi: 10.1099/00221287-129-4-1005. [DOI] [PubMed] [Google Scholar]
- Knowles C. J., Bunch A. W. Microbial cyanide metabolism. Adv Microb Physiol. 1986;27:73–111. doi: 10.1016/s0065-2911(08)60304-5. [DOI] [PubMed] [Google Scholar]
- Knowles C. J. Cyanide utilization and degradation by microorganisms. Ciba Found Symp. 1988;140:3–15. doi: 10.1002/9780470513712.ch2. [DOI] [PubMed] [Google Scholar]
- Kunz D. A., Nagappan O., Silva-Avalos J., Delong G. T. Utilization of cyanide as nitrogenous substrate by Pseudomonas fluorescens NCIMB 11764: evidence for multiple pathways of metabolic conversion. Appl Environ Microbiol. 1992 Jun;58(6):2022–2029. doi: 10.1128/aem.58.6.2022-2029.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kunz D. A., Wang C. S., Chen J. L. Alternative routes of enzymic cyanide metabolism in Pseudomonas fluorescens NCIMB 11764. Microbiology. 1994 Jul;140(Pt 7):1705–1712. doi: 10.1099/13500872-140-7-1705. [DOI] [PubMed] [Google Scholar]
- Shakir F. K., Audilet D., Drake A. J., 3rd, Shakir K. M. A rapid protein determination by modification of the Lowry procedure. Anal Biochem. 1994 Jan;216(1):232–233. doi: 10.1006/abio.1994.1031. [DOI] [PubMed] [Google Scholar]
- Silverman D. N., Tu C. K., Roessler N. Diffusion-limited exchange of 18O between CO2 and water in red cell suspensions. Respir Physiol. 1981 Jun;44(3):285–298. doi: 10.1016/0034-5687(81)90024-4. [DOI] [PubMed] [Google Scholar]