Abstract
The growth of a filamentous fungus, a Graphium sp., on n-alkanes (C(inf2) to C(inf4)) was inhibited by low concentrations of acetylene, propyne, 1-butyne, ethylene, and propylene. Acetylene and other unsaturated hydrocarbons had no effect on the growth of the Graphium sp. on potato dextrose broth, ethanol, or acetate. Our results suggest that n-alkynes and n-alkenes are selective inhibitors of a nonspecific monooxygenase enzyme responsible for the initial oxidation of n-alkanes.
Full Text
The Full Text of this article is available as a PDF (192.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Davies J. S., Wellman A. M., Zajic J. E. Hypomycetes utilizing natural gas. Can J Microbiol. 1973 Jan;19(1):81–85. doi: 10.1139/m73-012. [DOI] [PubMed] [Google Scholar]
- Davies J. S., Wellman A. M., Zajic J. E. Oxidation of ethane by an Acremonium species. Appl Environ Microbiol. 1976 Jul;32(1):14–20. doi: 10.1128/aem.32.1.14-20.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dilworth M. J. Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum. Biochim Biophys Acta. 1966 Oct 31;127(2):285–294. doi: 10.1016/0304-4165(66)90383-7. [DOI] [PubMed] [Google Scholar]
- Ensign S. A., Hyman M. R., Arp D. J. Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain. Appl Environ Microbiol. 1992 Sep;58(9):3038–3046. doi: 10.1128/aem.58.9.3038-3046.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hyman M. R., Arp D. J. Acetylene inhibition of metalloenzymes. Anal Biochem. 1988 Sep;173(2):207–220. doi: 10.1016/0003-2697(88)90181-9. [DOI] [PubMed] [Google Scholar]
- Hyman M. R., Wood P. M. Suicidal inactivation and labelling of ammonia mono-oxygenase by acetylene. Biochem J. 1985 May 1;227(3):719–725. doi: 10.1042/bj2270719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLee A. G., Kormendy A. C., Wayman M. Isolation and characterization of n-butane-utilizing microorganisms. Can J Microbiol. 1972 Aug;18(8):1191–1195. doi: 10.1139/m72-186. [DOI] [PubMed] [Google Scholar]
- Sariaslani F. S. Microbial cytochromes P-450 and xenobiotic metabolism. Adv Appl Microbiol. 1991;36:133–178. doi: 10.1016/s0065-2164(08)70453-2. [DOI] [PubMed] [Google Scholar]
- Volesky B., Zajic J. E. Batch production of protein from ethane and ethane-methane mixtures. Appl Microbiol. 1971 Apr;21(4):614–622. doi: 10.1128/am.21.4.614-622.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshinari T., Knowles R. Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria. Biochem Biophys Res Commun. 1976 Apr 5;69(3):705–710. doi: 10.1016/0006-291x(76)90932-3. [DOI] [PubMed] [Google Scholar]
- Zajic J. E., Volesky B., Wellman A. Growth of Graphium sp. on natural gas. Can J Microbiol. 1969 Oct;15(10):1231–1236. doi: 10.1139/m69-222. [DOI] [PubMed] [Google Scholar]
