Abstract
During the bioconversion of ricinoleic acid to (gamma)-decalactone under controlled pH conditions, Sporidiobolus salmonicolor produced only the lactone form, while Sporidiobolus ruinenii produced both the lactone form and a precursor. By using gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared analysis techniques, the precursor was identified as 4-hydroxydecanoic acid. The levels of production in the presence of high concentrations of ricinoleic acid methyl ester differed in the two Sporidiobolus species. This difference was due on the one hand to the high sensitivity of S. salmonicolor to the lactone and on the other hand to the high level of 4-hydroxydecanoic acid produced by S. ruinenii. 4-Hydroxydecanoic acid is much less toxic to the microorganisms than the lactone. In contrast to S. ruinenii, S. salmonicolor is not able to catabolize 4-hydroxydecanoic acid.
Full Text
The Full Text of this article is available as a PDF (248.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hagedorn S., Kaphammer B. Microbial biocatalysis in the generation of flavor and fragrance chemicals. Annu Rev Microbiol. 1994;48:773–800. doi: 10.1146/annurev.mi.48.100194.004013. [DOI] [PubMed] [Google Scholar]
- Jason A. C. A deterministic model for monophasic growth of batch cultures of bacteria. Antonie Van Leeuwenhoek. 1983 Dec;49(6):513–536. doi: 10.1007/BF00399845. [DOI] [PubMed] [Google Scholar]
- Neal A. L., Weinstock J. O., Lampen J. O. Mechanisms of Fatty Acid Toxicity for Yeast. J Bacteriol. 1965 Jul;90(1):126–131. doi: 10.1128/jb.90.1.126-131.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OKUI S., UCHIYAMA M., MIZUGAKI M. METABOLISM OF HYDROXY FATTY ACIDS. II. INTERMEDIATES OF THE OXIDATIVE BREAKDOWN OF RICINOLEIC ACID BY GENUS CANDIDA. J Biochem. 1963 Dec;54:536–540. doi: 10.1093/oxfordjournals.jbchem.a127827. [DOI] [PubMed] [Google Scholar]
- Spinnler H. E., Ginies C., Khan J. A., Vulfson E. N. Analysis of metabolic pathways by the growth of cells in the presence of organic solvents. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3373–3376. doi: 10.1073/pnas.93.8.3373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vanura H., Just M., Ambrosch F., Berger R. M., Bogaerts H., Wynen J., Vandevoorde D., Wiedermann G. Study of pertussis vaccines in infants: comparison of response to acellular pertussis DTP vaccines containing 25 micrograms of FHA and either 25 or 8 micrograms of PT with response to whole-cell pertussis DTP vaccine. Vaccine. 1994;12(3):210–214. doi: 10.1016/0264-410x(94)90196-1. [DOI] [PubMed] [Google Scholar]