Abstract
A fast, highly selective and sensitive method to quantify specific biomasses in mixed-culture biofilms is described. It consists of detachment of a biofilm from its support material, resolution of the detached biofilm flocs in order to separate the enclosed cells and antigens, and quantification of specific biomass by an enzyme-linked immunosorbent assay.
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- Baker K. H., Mills A. L. Determination of the Number of Respiring Thiobacillus ferrooxidans Cells in Water Samples by Using Combined Fluorescent Antibody-2-(p-Iodophenyl)-3-(p-Nitrophenyl)-5-Phenyltetrazolium Chloride Staining. Appl Environ Microbiol. 1982 Feb;43(2):338–344. doi: 10.1128/aem.43.2.338-344.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gorris M. T., Alarcon B., Lopez M. M., Cambra M. Characterization of Monoclonal Antibodies Specific for Erwinia carotovora subsp. atroseptica and Comparison of Serological Methods for Its Sensitive Detection on Potato Tubers. Appl Environ Microbiol. 1994 Jun;60(6):2076–2085. doi: 10.1128/aem.60.6.2076-2085.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoff K. A. Rapid and simple method for double staining of bacteria with 4',6-diamidino-2-phenylindole and fluorescein isothiocyanate-labeled antibodies. Appl Environ Microbiol. 1988 Dec;54(12):2949–2952. doi: 10.1128/aem.54.12.2949-2952.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee H. A., Wyatt G. M., Bramham S., Morgan M. R. Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection. Appl Environ Microbiol. 1990 Jun;56(6):1541–1546. doi: 10.1128/aem.56.6.1541-1546.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neumann A., Scholz-Muramatsu H., Diekert G. Tetrachloroethene metabolism of Dehalospirillum multivorans. Arch Microbiol. 1994;162(4):295–301. doi: 10.1007/BF00301854. [DOI] [PubMed] [Google Scholar]
- Nybroe O., Johansen A., Laake M. Enzyme-linked immunosorbent assays for detection of Pseudomonas fluorescens in sediment samples. Lett Appl Microbiol. 1990 Dec;11(6):293–296. doi: 10.1111/j.1472-765x.1990.tb00185.x. [DOI] [PubMed] [Google Scholar]
- Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
- Saraswat N., Alleman J. E., Smith T. J. Enzyme Immunoassay Detection of Nitrosomonas europaea. Appl Environ Microbiol. 1994 Jun;60(6):1969–1973. doi: 10.1128/aem.60.6.1969-1973.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaule G., Flemming H. C., Ridgway H. F. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl Environ Microbiol. 1993 Nov;59(11):3850–3857. doi: 10.1128/aem.59.11.3850-3857.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turakhia M. H., Cooksey K. E., Characklis W. G. Influence of a calcium-specific chelant on biofilm removal. Appl Environ Microbiol. 1983 Nov;46(5):1236–1238. doi: 10.1128/aem.46.5.1236-1238.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward B. B., Perry M. J. Immunofluorescent Assay for the Marine Ammonium-Oxidizing Bacterium Nitrosococcus oceanus. Appl Environ Microbiol. 1980 Apr;39(4):913–918. doi: 10.1128/aem.39.4.913-918.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyatt G. M., Langley M. N., Lee H. A., Morgan M. R. Further studies on the feasibility of one-day Salmonella detection by enzyme-linked immunosorbent assay. Appl Environ Microbiol. 1993 May;59(5):1383–1390. doi: 10.1128/aem.59.5.1383-1390.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]