Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1996 Sep;62(9):3483–3485. doi: 10.1128/aem.62.9.3483-3485.1996

Methanogenic Transformation of Methylfurfural Compounds to Furfural

R Boopathy
PMCID: PMC1388948  PMID: 16535410

Abstract

The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabolized, almost 1 mol of furfural and 0.7 mol of methane were produced. Several methanogenic bacteria did not carry out this conversion. The metabolic conversion of methylfurfurals is likely to be of value in the anaerobic treatment of methylfurfural-containing wastewaters such as those produced by the paper and pulp industries and oatmeal processing industries. This study adds to the list of the limited number of compounds that are known to serve as electron donors for methanogenesis.

Full Text

The Full Text of this article is available as a PDF (174.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdulrashid N., Clark D. P. Isolation and genetic analysis of mutations allowing the degradation of furans and thiophenes by Escherichia coli. J Bacteriol. 1987 Mar;169(3):1267–1271. doi: 10.1128/jb.169.3.1267-1271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belay N., Daniels L. Production of ethane, ethylene, and acetylene from halogenated hydrocarbons by methanogenic bacteria. Appl Environ Microbiol. 1987 Jul;53(7):1604–1610. doi: 10.1128/aem.53.7.1604-1610.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boopathy R., Kulpa C. F. Biotransformation of 2,4,6-trinitrotoluene (TNT) by a Methanococcus sp. (strain B) isolated from a lake sediment. Can J Microbiol. 1994 Apr;40(4):273–278. doi: 10.1139/m94-044. [DOI] [PubMed] [Google Scholar]
  5. Brune G., Schoberth S. M., Sahm H. Growth of a strictly anaerobic bacterium on furfural (2-furaldehyde). Appl Environ Microbiol. 1983 Nov;46(5):1187–1192. doi: 10.1128/aem.46.5.1187-1192.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daniels L., Belay N., Rajagopal B. S. Assimilatory reduction of sulfate and sulfite by methanogenic bacteria. Appl Environ Microbiol. 1986 Apr;51(4):703–709. doi: 10.1128/aem.51.4.703-709.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferry J. G. Biochemistry of methanogenesis. Crit Rev Biochem Mol Biol. 1992;27(6):473–503. doi: 10.3109/10409239209082570. [DOI] [PubMed] [Google Scholar]
  8. Koenig K., Andreesen J. R. Molybdenum Involvement in Aerobic Degradation of 2-Furoic Acid by Pseudomonas putida Fu1. Appl Environ Microbiol. 1989 Jul;55(7):1829–1834. doi: 10.1128/aem.55.7.1829-1834.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Brien J. M., Wolkin R. H., Moench T. T., Morgan J. B., Zeikus J. G. Association of hydrogen metabolism with unitrophic or mixotrophic growth of Methanosarcina barkeri on carbon monoxide. J Bacteriol. 1984 Apr;158(1):373–375. doi: 10.1128/jb.158.1.373-375.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oremland R. S., Kiene R. P., Mathrani I., Whiticar M. J., Boone D. R. Description of an estuarine methylotrophic methanogen which grows on dimethyl sulfide. Appl Environ Microbiol. 1989 Apr;55(4):994–1002. doi: 10.1128/aem.55.4.994-1002.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wackett L. P., Honek J. F., Begley T. P., Wallace V., Orme-Johnson W. H., Walsh C. T. Substrate analogues as mechanistic probes of methyl-S-coenzyme M reductase. Biochemistry. 1987 Sep 22;26(19):6012–6018. doi: 10.1021/bi00393a010. [DOI] [PubMed] [Google Scholar]
  12. van der Maarel M., Jansen M., Hansen T. A. Methanogenic conversion of 3-s-methylmercaptopropionate to 3-mercaptopropionate. Appl Environ Microbiol. 1995 Jan;61(1):48–51. doi: 10.1128/aem.61.1.48-51.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES