Abstract
The disappearance of stable-isotope-labeled formate in freshwater sediment from Lake Vechten was studied by gas chromatography-mass spectrometry. Both deuterium- and (sup13)C-labeled formate were removed from pore water at a high rate (400 (plusmn) 35 [standard deviation] and 1,700 (plusmn) 600 (mu)M h(sup-1), respectively), with concomitant formation of nonlabeled formate. The removal rate of labeled and nonlabeled formate amounted to 240 (plusmn) 15 and 400 (plusmn) 40 (mu)M h(sup-1), respectively. The formation of nonlabeled formate is explained by isotopic exchange due to the activity of formate dehydrogenase rather than by the turnover of formate.
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Selected References
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- Boone D. R., Johnson R. L., Liu Y. Diffusion of the Interspecies Electron Carriers H(2) and Formate in Methanogenic Ecosystems and Its Implications in the Measurement of K(m) for H(2) or Formate Uptake. Appl Environ Microbiol. 1989 Jul;55(7):1735–1741. doi: 10.1128/aem.55.7.1735-1741.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cappenberg T. E., Prins R. A. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. 3. Experiments with 14C-labeled substrates. Antonie Van Leeuwenhoek. 1974;40(3):457–469. doi: 10.1007/BF00399358. [DOI] [PubMed] [Google Scholar]
- Guyot J. P., Brauman A. Methane Production from Formate by Syntrophic Association of Methanobacterium bryantii and Desulfovibrio vulgaris JJ. Appl Environ Microbiol. 1986 Dec;52(6):1436–1437. doi: 10.1128/aem.52.6.1436-1437.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovley D. R., Klug M. J. Intermediary metabolism of organic matter in the sediments of a eutrophic lake. Appl Environ Microbiol. 1982 Mar;43(3):552–560. doi: 10.1128/aem.43.3.552-560.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stams A. J. Metabolic interactions between anaerobic bacteria in methanogenic environments. Antonie Van Leeuwenhoek. 1994;66(1-3):271–294. doi: 10.1007/BF00871644. [DOI] [PubMed] [Google Scholar]
- Sørensen J., Christensen D., Jørgensen B. B. Volatile Fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl Environ Microbiol. 1981 Jul;42(1):5–11. doi: 10.1128/aem.42.1.5-11.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Graaf W., Wellsbury P., Parkes R. J., Cappenberg T. E. Comparison of acetate turnover in methanogenic and sulfate-reducing sediments by radiolabeling and stable isotope labeling and by use of specific inhibitors: evidence for isotopic exchange. Appl Environ Microbiol. 1996 Mar;62(3):772–777. doi: 10.1128/aem.62.3.772-777.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]