Abstract
Phylogenetic and phenotypic analysis indicates that a moderately thermophilic isolate, C-SH12, from Australia belongs to the species Thiobacillus caldus. Antiserum generated against whole cells of T. caldus KU recognized protein antigens common to cell lysates of the three T. caldus strains KU, BC13, and C-SH12 but did not recognize whole cells of isolate C-SH12. Differences in the lipopolysaccharide (LPS) of strain C-SH12 and those of the other two T. caldus strains were found, and the anti-KU antiserum did not recognize the LPS from strain C-SH12. These data indicate that this T. caldus isolate belongs to a serotype different from that of strains KU and BC13.
Full Text
The Full Text of this article is available as a PDF (298.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amaro A. M., Hallberg K. B., Lindström E. B., Jerez C. A. An immunological assay for detection and enumeration of thermophilic biomining microorganisms. Appl Environ Microbiol. 1994 Sep;60(9):3470–3473. doi: 10.1128/aem.60.9.3470-3473.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Arredondo Renato, Jerez Carlos A. Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans. Appl Environ Microbiol. 1989 Aug;55(8):2025–2029. doi: 10.1128/aem.55.8.2025-2029.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goebel B. M., Stackebrandt E. Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. Appl Environ Microbiol. 1994 May;60(5):1614–1621. doi: 10.1128/aem.60.5.1614-1621.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hallberg K. B., Lindström E. B. Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile. Microbiology. 1994 Dec;140(Pt 12):3451–3456. doi: 10.1099/13500872-140-12-3451. [DOI] [PubMed] [Google Scholar]
- Harrison A. P., Jr The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. Annu Rev Microbiol. 1984;38:265–292. doi: 10.1146/annurev.mi.38.100184.001405. [DOI] [PubMed] [Google Scholar]
- Higgins D. G. CLUSTAL V: multiple alignment of DNA and protein sequences. Methods Mol Biol. 1994;25:307–318. doi: 10.1385/0-89603-276-0:307. [DOI] [PubMed] [Google Scholar]
- Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hitchcock P. J., Leive L., Mäkelä P. H., Rietschel E. T., Strittmatter W., Morrison D. C. Lipopolysaccharide nomenclature--past, present, and future. J Bacteriol. 1986 Jun;166(3):699–705. doi: 10.1128/jb.166.3.699-705.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Macke T. J., Marsh T. L., Woese C. R. The ribosomal database project. Nucleic Acids Res. 1993 Jul 1;21(13):3021–3023. doi: 10.1093/nar/21.13.3021. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muyzer G., de Bruyn A. C., Schmedding D. J., Bos P., Westbroek P., Kuenen G. J. A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of Thiobacillus ferrooxidans in a Population of Acidophilic Bacteria. Appl Environ Microbiol. 1987 Apr;53(4):660–664. doi: 10.1128/aem.53.4.660-664.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
- Southam G., Beveridge T. J. Examination of Lipopolysaccharide (O-Antigen) Populations of Thiobacillus ferrooxidans from Two Mine Tailings. Appl Environ Microbiol. 1993 May;59(5):1283–1288. doi: 10.1128/aem.59.5.1283-1288.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]