Abstract
A chemically defined medium for the growth of Leuconostoc mesenteroides was developed. This medium contained lactose, Mn(sup2+), Mg(sup2+), 12 amino acids, eight vitamins, adenine, uracil, and Tween 80. We showed the beneficial effect of aerobic conditions on growth and that potassium phosphate (135 mM) is a suitable buffer. The growth rate in this medium was 0.85 (plusmn) 0.10 h(sup-1) for the six strains examined, and cell densities up to 3.5 x 10(sup9) CFU/ml were attained.
Full Text
The Full Text of this article is available as a PDF (184.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bohonos N., Hutchings B. L., Peterson W. H. Pyridoxine Nutrition of Lactic Acid Bacteria. J Bacteriol. 1942 Oct;44(4):479–485. doi: 10.1128/jb.44.4.479-485.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douglas J. A critical review of the use of glycerophosphates in microbiological media. Lab Pract. 1971 May;20(5):414–passim. [PubMed] [Google Scholar]
- Gaines S., Stahly G. L. The Growth Requirements of Leuconostoc Mesenteroides and Preliminary Studies on its Use as an Assay Agent for Several Members of the Vitamin B Complex. J Bacteriol. 1943 Nov;46(5):441–449. doi: 10.1128/jb.46.5.441-449.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garvie E. I. The growth factor and amino acid requirements of species of the genus Leuconostoc, including Leuconostoc paramesenteroides (sp.nov.) and Leuconostoc oenos. J Gen Microbiol. 1967 Sep;48(3):439–447. doi: 10.1099/00221287-48-3-439. [DOI] [PubMed] [Google Scholar]
- Jensen P. R., Hammer K. Minimal Requirements for Exponential Growth of Lactococcus lactis. Appl Environ Microbiol. 1993 Dec;59(12):4363–4366. doi: 10.1128/aem.59.12.4363-4366.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poolman B., Konings W. N. Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport. J Bacteriol. 1988 Feb;170(2):700–707. doi: 10.1128/jb.170.2.700-707.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROGOSA M., FRANKLIN J. G., PERRY K. D. Correlation of the vitamin requirements with cultural and biochemical characters of Lactobacillus spp. J Gen Microbiol. 1961 Jul;25:473–482. doi: 10.1099/00221287-25-3-473. [DOI] [PubMed] [Google Scholar]
- Sneli E. E., Strong F. M., Peterson W. H. Growth Factors for Bacteria: VIII. Pantothenic and Nicotinic Acids as Essential Growth Factors for Lactic and Propionic Acid Bacteria. J Bacteriol. 1939 Sep;38(3):293–308. doi: 10.1128/jb.38.3.293-308.1939. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snell E. E., Mitchell H. K. Purine and Pyrimidine as Growth Substances for Lactic Acid Bacteria. Proc Natl Acad Sci U S A. 1941 Jan 15;27(1):1–7. doi: 10.1073/pnas.27.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITESIDE-CARLSON V., ROSANO C. L. The nutritional requirements of Leuconostoc dextranicum for growth and dextran synthesis. J Bacteriol. 1951 Nov;62(5):583–589. doi: 10.1128/jb.62.5.583-589.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whiteside-Carlson V., Carlson W. W. THE VITAMIN REQUIREMENTS OF LEUCONOSTOC FOR DEXTRAN SYNTHESIS. J Bacteriol. 1949 Aug;58(2):135–141. doi: 10.1128/jb.58.2.135-141.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]