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. 1997 Jul;63(7):2660–2664. doi: 10.1128/aem.63.7.2660-2664.1997

Efficiency of Indigenous and Inoculated Cold-Adapted Soil Microorganisms for Biodegradation of Diesel Oil in Alpine Soils

R Margesin, F Schinner
PMCID: PMC1389197  PMID: 16535642

Abstract

Biodegradation of diesel oil (5 g(middot)kg [soil dry weight](sup-1)) was investigated in five alpine subsoils, differing in soil type and bedrock, in laboratory experiments during 20 days at 10(deg)C. The biodegradation activities of the indigenous soil microorganisms and of a psychrotrophic diesel oil-degrading inoculum and the effect of biostimulation by inorganic fertilization (C/N/P ratio = 100:10:2) were determined. Fertilization significantly enhanced diesel oil biodegradation activity of the indigenous soil microorganisms. Biostimulation by fertilization enhanced diesel oil biodegradation to a significantly greater degree than bioaugmentation with the psychrotrophic inoculum. In none of the five soils did fertilization plus inoculation result in a higher decontamination than fertilization alone. A total of 16 to 23% of the added diesel oil contamination was lost by abiotic processes. Total decontamination without and with fertilization was in the range of 16 to 31 and 27 to 53%, respectively.

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Selected References

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  1. Dorn E., Hellwig M., Reineke W., Knackmuss H. J. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974;99(1):61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
  2. Goldstein R. M., Mallory L. M., Alexander M. Reasons for possible failure of inoculation to enhance biodegradation. Appl Environ Microbiol. 1985 Oct;50(4):977–983. doi: 10.1128/aem.50.4.977-983.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gounot A. M. Bacterial life at low temperature: physiological aspects and biotechnological implications. J Appl Bacteriol. 1991 Nov;71(5):386–397. doi: 10.1111/j.1365-2672.1991.tb03806.x. [DOI] [PubMed] [Google Scholar]
  4. Grem J. L., van Groeningen C. J., Ismail A. A., Johnston P. G., Alexander H. R., Allegra C. J. The role of interferon-alpha as a modulator of fluorouracil and leucovorin. Eur J Cancer. 1995 Jul-Aug;31A(7-8):1316–1320. doi: 10.1016/0959-8049(95)91267-g. [DOI] [PubMed] [Google Scholar]
  5. Lindstrom J. E., Prince R. C., Clark J. C., Grossman M. J., Yeager T. R., Braddock J. F., Brown E. J. Microbial populations and hydrocarbon biodegradation potentials in fertilized shoreline sediments affected by the T/V Exxon Valdez oil spill. Appl Environ Microbiol. 1991 Sep;57(9):2514–2522. doi: 10.1128/aem.57.9.2514-2522.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morgan P., Watkinson R. J. Hydrocarbon degradation in soils and methods for soil biotreatment. Crit Rev Biotechnol. 1989;8(4):305–333. doi: 10.3109/07388558909148196. [DOI] [PubMed] [Google Scholar]
  7. Morita R. Y. Psychrophilic bacteria. Bacteriol Rev. 1975 Jun;39(2):144–167. doi: 10.1128/br.39.2.144-167.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Møller J., Gaarn H., Steckel T., Wedebye E. B., Westermann P. Inhibitory effects on degradation of diesel oil in soil-microcosms by a commercial bioaugmentation product. Bull Environ Contam Toxicol. 1995 Jun;54(6):913–918. doi: 10.1007/BF00197978. [DOI] [PubMed] [Google Scholar]
  9. Russell N. J. Cold adaptation of microorganisms. Philos Trans R Soc Lond B Biol Sci. 1990 Jan 30;326(1237):595-608, discussion 608-11. doi: 10.1098/rstb.1990.0034. [DOI] [PubMed] [Google Scholar]
  10. Swindoll C. M., Aelion C. M., Pfaender F. K. Influence of inorganic and organic nutrients on aerobic biodegradation and on the adaptation response of subsurface microbial communities. Appl Environ Microbiol. 1988 Jan;54(1):212–217. doi: 10.1128/aem.54.1.212-217.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Whyte L. G., Greer C. W., Inniss W. E. Assessment of the biodegradation potential of psychrotrophic microorganisms. Can J Microbiol. 1996 Feb;42(2):99–106. doi: 10.1139/m96-016. [DOI] [PubMed] [Google Scholar]
  12. van der Walt J. P., von Arx J. A. The yeast genus Yarrowia gen. nov. Antonie Van Leeuwenhoek. 1980;46(6):517–521. doi: 10.1007/BF00394008. [DOI] [PubMed] [Google Scholar]

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