Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1997 Jul;63(7):2910–2912. doi: 10.1128/aem.63.7.2910-2912.1997

In Vitro Interactions of Asian Freshwater Clam (Corbicula fluminea) Hemocytes and Cryptosporidium parvum Oocysts

T K Graczyk, R Fayer, M R Cranfield, D B Conn
PMCID: PMC1389211  PMID: 16535656

Abstract

Corbicula fluminea hemocytes phagocytosed infectious oocysts of Cryptosporidium parvum in vitro. After 15, 30, 60, 90, and 120 min of incubation, averages of 35.8, 58.0, 69.7, 77.7, and 81.6% of the oocysts were phagocytosed by 24.3, 70.0, 78.5, 87.3, and 93.0% of the hemocytes, respectively. A single clam can retain by phagocytosis an average of 1.84 x 10(sup6) oocysts per ml of hemolymph. C. fluminea bivalves can serve as biological indicators of contamination of wastewaters and agricultural drainages with Cryptosporidium.

Full Text

The Full Text of this article is available as a PDF (228.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fayer R. Effect of high temperature on infectivity of Cryptosporidium parvum oocysts in water. Appl Environ Microbiol. 1994 Aug;60(8):2732–2735. doi: 10.1128/aem.60.8.2732-2735.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fayer R., Ellis W. Paromomycin is effective as prophylaxis for cryptosporidiosis in dairy calves. J Parasitol. 1993 Oct;79(5):771–774. [PubMed] [Google Scholar]
  3. Fayer R., Farley C. A., Lewis E. J., Trout J. M., Graczyk T. K. Potential Role of the Eastern Oyster, Crassostrea virginica, in the Epidemiology of Cryptosporidium parvum. Appl Environ Microbiol. 1997 May;63(5):2086–2088. doi: 10.1128/aem.63.5.2086-2088.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fried B., Emili S., Ettinger W. S. Experimental infection of Corbicula fluminea (Bivalvia: Corbiculidae) with Echinostoma revolutum cercariae. J Parasitol. 1987 Jun;73(3):655–656. [PubMed] [Google Scholar]
  5. Graczyk T. K., Cranfield M. R., Fayer R. Evaluation of commercial enzyme immunoassay (EIA) and immunofluorescent antibody (FA) test kits for detection of Cryptosporidium oocysts of species other than Cryptosporidium parvum. Am J Trop Med Hyg. 1996 Mar;54(3):274–279. doi: 10.4269/ajtmh.1996.54.274. [DOI] [PubMed] [Google Scholar]
  6. Kira S., Nogami Y., Taketa K., Hayatsu H. Comparison of techniques for monitoring water-borne polycyclic mutagens: efficiency of blue rayon, Sep-Pak C18, and a biota, Corbicula, in concentrating benzo(a)pyrene in a model water system. Bull Environ Contam Toxicol. 1996 Aug;57(2):278–283. doi: 10.1007/s001289900187. [DOI] [PubMed] [Google Scholar]
  7. Kramer M. H., Herwaldt B. L., Craun G. F., Calderon R. L., Juranek D. D. Surveillance for waterborne-disease outbreaks--United States, 1993-1994. MMWR CDC Surveill Summ. 1996 Apr 12;45(1):1–33. [PubMed] [Google Scholar]
  8. Kumazawa N. H., Morimoto N. Chemotactic activity of hemocytes derived from a brackish-water clam, Corbicula japonica, to Vibrio parahaemolyticus and Escherichia coli strains. J Vet Med Sci. 1992 Oct;54(5):851–855. doi: 10.1292/jvms.54.851. [DOI] [PubMed] [Google Scholar]
  9. Leland H. V., Scudder B. C. Trace elements in Corbicula fluminea from the San Joaquin River, California. Sci Total Environ. 1990 Nov;97-98:641–672. doi: 10.1016/0048-9697(90)90267-x. [DOI] [PubMed] [Google Scholar]
  10. Tatem H. E. Bioaccumulation of polychlorinated biphenyls and metals from contaminated sediment by freshwater prawns, Macrobrachium rosenbergii and clams, Corbicula fluminea. Arch Environ Contam Toxicol. 1986 Feb;15(2):171–183. doi: 10.1007/BF01059966. [DOI] [PubMed] [Google Scholar]
  11. Winger P. V., Sieckman C., May T. W., Johnson W. W. Residues of organochlorine insecticides, polychlorinated biphenyls, and heavy metals in biota from Apalachicola River, Florida, 1978. J Assoc Off Anal Chem. 1984 Mar-Apr;67(2):325–333. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES