Abstract
Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b. All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O(inf2). Butane inhibited CF degradation by the butane-grown bacteria, suggesting that butane monooxygenase is responsible for CF degradation. P. butanovora required exogenous reductant to degrade CF, while CF8 and M. vaccae utilized endogenous reductants. Prolonged incubation with CF resulted in decreased CF degradation. CF8 and P. butanovora were more sensitive to CF than either M. trichosporium or M. vaccae. CF degradation by all three butane-grown bacteria was inactivated by acetylene, which is a mechanism-based inhibitor for several monooxygenases. Butane protected all three butane-grown bacteria from inactivation by acetylene, which indicates that the same monooxygenase is responsible for both CF and butane oxidation. CF8 and P. butanovora were able to degrade other chlorinated hydrocarbons, including trichloroethylene, 1,2-cis-dichloroethylene, and vinyl chloride. In addition, CF8 degraded 1,1,2-trichloroethane. The results indicate the potential of butane-grown bacteria for chlorinated hydrocarbon transformation.
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- Alvarez-Cohen L., McCarty P. L. Product toxicity and cometabolic competitive inhibition modeling of chloroform and trichloroethylene transformation by methanotrophic resting cells. Appl Environ Microbiol. 1991 Apr;57(4):1031–1037. doi: 10.1128/aem.57.4.1031-1037.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolt H. M., Filser J. G. Irreversible binding of chlorinated ethylenes to macromolecules. Environ Health Perspect. 1977 Dec;21:107–112. doi: 10.1289/ehp.7721107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bédard C., Knowles R. Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers. Microbiol Rev. 1989 Mar;53(1):68–84. doi: 10.1128/mr.53.1.68-84.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang H. L., Alvarez-Cohen L. Biodegradation of individual and multiple chlorinated aliphatic hydrocarbons by methane-oxidizing cultures. Appl Environ Microbiol. 1996 Sep;62(9):3371–3377. doi: 10.1128/aem.62.9.3371-3377.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McClay K., Fox B. G., Steffan R. J. Chloroform mineralization by toluene-oxidizing bacteria. Appl Environ Microbiol. 1996 Aug;62(8):2716–2722. doi: 10.1128/aem.62.8.2716-2722.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oldenhuis R., Oedzes J. Y., van der Waarde J. J., Janssen D. B. Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene. Appl Environ Microbiol. 1991 Jan;57(1):7–14. doi: 10.1128/aem.57.1.7-14.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oldenhuis R., Vink R. L., Janssen D. B., Witholt B. Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase. Appl Environ Microbiol. 1989 Nov;55(11):2819–2826. doi: 10.1128/aem.55.11.2819-2826.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips W. E., Jr, Perry J. J. Metabolism of n-butane and 2-butanone by Mycobacterium vaccae. J Bacteriol. 1974 Nov;120(2):987–989. doi: 10.1128/jb.120.2.987-989.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pohl L. R., Bhooshan B., Whittaker N. F., Krishna G. Phosgene: a metabolite of chloroform. Biochem Biophys Res Commun. 1977 Dec 7;79(3):684–691. doi: 10.1016/0006-291x(77)91166-4. [DOI] [PubMed] [Google Scholar]
- Rasche M. E., Hicks R. E., Hyman M. R., Arp D. J. Oxidation of monohalogenated ethanes and n-chlorinated alkanes by whole cells of Nitrosomonas europaea. J Bacteriol. 1990 Sep;172(9):5368–5373. doi: 10.1128/jb.172.9.5368-5373.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rasche M. E., Hyman M. R., Arp D. J. Factors Limiting Aliphatic Chlorocarbon Degradation by Nitrosomonas europaea: Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity. Appl Environ Microbiol. 1991 Oct;57(10):2986–2994. doi: 10.1128/aem.57.10.2986-2994.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsien H. C., Brusseau G. A., Hanson R. S., Waclett L. P. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl Environ Microbiol. 1989 Dec;55(12):3155–3161. doi: 10.1128/aem.55.12.3155-3161.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vannelli T., Logan M., Arciero D. M., Hooper A. B. Degradation of halogenated aliphatic compounds by the ammonia- oxidizing bacterium Nitrosomonas europaea. Appl Environ Microbiol. 1990 Apr;56(4):1169–1171. doi: 10.1128/aem.56.4.1169-1171.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vestal J. R., Perry J. J. Divergent metabolic pathways for propane and propionate utilization by a soil isolate. J Bacteriol. 1969 Jul;99(1):216–221. doi: 10.1128/jb.99.1.216-221.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wackett L. P., Brusseau G. A., Householder S. R., Hanson R. S. Survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Appl Environ Microbiol. 1989 Nov;55(11):2960–2964. doi: 10.1128/aem.55.11.2960-2964.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wackett L. P., Gibson D. T. Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1. Appl Environ Microbiol. 1988 Jul;54(7):1703–1708. doi: 10.1128/aem.54.7.1703-1708.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]