Abstract
To investigate the possible cometabolic biodegradation of benzo[a]pyrene (BaP), crude oil spiked with [7-(sup14)C]BaP and unlabeled BaP was added to soil with no known pollution history, to give 34 g of oil and 67 mg of BaP/kg of dry soil. The oil-soil mixture was amended with mineral nutrients and incubated in an airtight container with continuous forced aeration. Total CO(inf2) and (sup14)CO(inf2) in the off-gas were trapped and quantified. Soil samples were Soxhlet extracted with dichloromethane at seven time points during the 150-day incubation period, and the extracted soil was subjected to further fractionation in order to recover reversibly and irreversibly bound radiocarbon. Radiocarbon recovery was 100% (plusmn) 3% for each time point. During the first 50 days of incubation, no (sup14)CO(inf2) was evolved, but over the next 100 days, 50% of the BaP radiocarbon was evolved as (sup14)CO(inf2). At 150 days, only 5% of the intact BaP and 23% of the crude oil remained. Of the remaining radiolabel, 20% was found in solvent-extractable metabolites and 25% was incorporated into soil organic matter. Only 1/10 of this could be solubilized by chemical hydrolysis. An abiotic control experiment exhibited binding of only 2% of the BaP, indicating the microbial nature of the BaP transformations. We report that in soil containing suitable cosubstrates, BaP can be completely degraded.
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Selected References
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- Cerniglia C. E., Gibson D. T. Oxidation of benzo[a]pyrene by the filamentous fungus Cunninghamella elegans. J Biol Chem. 1979 Dec 10;254(23):12174–12180. [PubMed] [Google Scholar]
- Cerniglia C. E., Mahaffey W., Gibson D. T. Fungal oxidation of benzo[a]pyrene: formation of (-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene by Cunninghamella elegans. Biochem Biophys Res Commun. 1980 May 14;94(1):226–232. doi: 10.1016/s0006-291x(80)80210-5. [DOI] [PubMed] [Google Scholar]
- Datta D., Samanta T. B. Effect of inducers on metabolism of benzo(a)pyrene in vivo and in vitro: analysis by high pressure liquid chromatography. Biochem Biophys Res Commun. 1988 Aug 30;155(1):493–502. doi: 10.1016/s0006-291x(88)81114-8. [DOI] [PubMed] [Google Scholar]
- Fogel M. M., Taddeo A. R., Fogel S. Biodegradation of chlorinated ethenes by a methane-utilizing mixed culture. Appl Environ Microbiol. 1986 Apr;51(4):720–724. doi: 10.1128/aem.51.4.720-724.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibson D. T., Mahadevan V., Jerina D. M., Yogi H., Yeh H. J. Oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium. Science. 1975 Jul 25;189(4199):295–297. doi: 10.1126/science.1145203. [DOI] [PubMed] [Google Scholar]
- Grosser R. J., Warshawsky D., Vestal J. R. Indigenous and enhanced mineralization of pyrene, benzo[a]pyrene, and carbazole in soils. Appl Environ Microbiol. 1991 Dec;57(12):3462–3469. doi: 10.1128/aem.57.12.3462-3469.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haemmerli S. D., Leisola M. S., Sanglard D., Fiechter A. Oxidation of benzo(a)pyrene by extracellular ligninases of Phanerochaete chrysosporium. Veratryl alcohol and stability of ligninase. J Biol Chem. 1986 May 25;261(15):6900–6903. [PubMed] [Google Scholar]
- Jimenez I. Y., Bartha R. Solvent-Augmented Mineralization of Pyrene by a Mycobacterium sp. Appl Environ Microbiol. 1996 Jul;62(7):2311–2316. doi: 10.1128/aem.62.7.2311-2316.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schneider J., Grosser R., Jayasimhulu K., Xue W., Warshawsky D. Degradation of pyrene, benz[a]anthracene, and benzo[a]pyrene by Mycobacterium sp. strain RJGII-135, isolated from a former coal gasification site. Appl Environ Microbiol. 1996 Jan;62(1):13–19. doi: 10.1128/aem.62.1.13-19.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shuttlesworth KL, Cerniglia CE. Bacterial Degradation of Low Concentrations of Phenanthrene and Inhibition by Naphthalene. Microb Ecol. 1996 May;31(3):305–317. [PubMed] [Google Scholar]
- Stringfellow W. T., Aitken M. D. Competitive metabolism of naphthalene, methylnaphthalenes, and fluorene by phenanthrene-degrading pseudomonads. Appl Environ Microbiol. 1995 Jan;61(1):357–362. doi: 10.1128/aem.61.1.357-362.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]