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. 1997 Dec;63(12):4993–4995. doi: 10.1128/aem.63.12.4993-4995.1997

A Simple, Efficient Method for the Separation of Humic Substances and DNA from Environmental Samples

C R Jackson, J P Harper, D Willoughby, E E Roden, P F Churchill
PMCID: PMC1389316  PMID: 16535760

Abstract

Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.

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Selected References

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