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. 1971 Jan;212(2):503–517. doi: 10.1113/jphysiol.1971.sp009338

On the control of glycogenolysis in mammalian nervous tissue by calcium

D Landowne, J M Ritchie
PMCID: PMC1395658  PMID: 4323308

Abstract

1. A study has been made of the increase in fluorescence of the desheathed cervical vagus nerve that occurs after electrical stimulation (usually 5 sec at 30/sec) of its non-myelinated fibres.

2. At room temperature this increase in fluorescence is normally masked by the decrease in fluorescence caused by mitochondrial oxidative phosphorylation. However, at higher temperatures (30-35° C) the increasing fluorescence phase predominates and the net change on stimulation is an increase.

3. At room temperature the increase in fluorescence is seen clearly only when ATP splitting has been prevented by ouabain, by bathing the nerve in lithium-Locke solution, or when oxidative phosphorylation has been prevented by metabolic inhibitors.

4. The increasing fluorescence response is absent when calcium is removed from the external medium; it increases with increasing calcium concentration.

5. It is argued that the increasing fluorescence response is due to an increase in glycogenolysis (leading to an increase in the reduced pyridine nucleotide concentration) brought about by the increased calcium entry during the action potential. This calcium presumably increases the activity of phosphorylates a or phosphofructokinase.

6. Calcium entry also speeds mitochondrial oxidative phosphorylation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armett C. J., Ritchie J. M. On the permeability of mammalian non-myelinated fibres to sodium and to lithium ions. J Physiol. 1963 Jan;165(1):130–140. doi: 10.1113/jphysiol.1963.sp007047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker P. F., Hodgkin A. L., Ridgway E. B. Two phases of calcium entry during the action potential in giant axons of Loligo. J Physiol. 1970 Jun;208(2):80P–82P. [PubMed] [Google Scholar]
  3. CHANCE B. THE ENERGY-LINKED REACTION OF CALCIUM WITH MITOCHONDRIA. J Biol Chem. 1965 Jun;240:2729–2748. [PubMed] [Google Scholar]
  4. DANFORTH W. H., HELMREICH E. REGULATION OF GLYCOLYSIS IN MUSCLE. I. THE CONVERSION OF PHOSPHORYLASE BETA TO PHOSPHORYLASE ALPHA IN FROG SARTORIUS MUSCLE. J Biol Chem. 1964 Oct;239:3133–3138. [PubMed] [Google Scholar]
  5. GREENGARD P., STRAUB R. W. Effect of frequency of electrical stimulation on the concentration of intermediary metabolites in mammalian non-myelinated fibres. J Physiol. 1959 Oct;148:353–361. doi: 10.1113/jphysiol.1959.sp006292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HODGKIN A. L., KEYNES R. D. Movements of labelled calcium in squid giant axons. J Physiol. 1957 Sep 30;138(2):253–281. doi: 10.1113/jphysiol.1957.sp005850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KREBS E. G., GRAVES D. J., FISCHER E. H. Factors affecting the activity of muscle phosphorylase b kinase. J Biol Chem. 1959 Nov;234:2867–2873. [PubMed] [Google Scholar]
  8. Keynes R. D., Ritchie J. M. The movements of labelled ions in mammalian non-myelinated nerve fibres. J Physiol. 1965 Jul;179(2):333–367. doi: 10.1113/jphysiol.1965.sp007666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., PASSONNEAU J. V., HASSELBERGER F. X., SCHULZ D. W. EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN. J Biol Chem. 1964 Jan;239:18–30. [PubMed] [Google Scholar]
  10. LOWRY O. H., PASSONNEAU J. V. THE RELATIONSHIPS BETWEEN SUBSTRATES AND ENZYMES OF GLYCOLYSIS IN BRAIN. J Biol Chem. 1964 Jan;239:31–42. [PubMed] [Google Scholar]
  11. Landowne D., Ritchie J. M. Optical studies on the kinetics of the sodium pump in mammalian non-myelinated nerve fibres. J Physiol. 1971 Jan;212(2):483–502. doi: 10.1113/jphysiol.1971.sp009337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rang H. P., Ritchie J. M. On the electrogenic sodium pump in mammalian non-myelinated nerve fibres and its activation by various external cations. J Physiol. 1968 May;196(1):183–221. doi: 10.1113/jphysiol.1968.sp008502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rang H. P., Ritchie J. M. The ionic content of mammalian non-myelinated nerve fibres and its alteration as a result of electrical activity. J Physiol. 1968 May;196(1):223–236. doi: 10.1113/jphysiol.1968.sp008503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rodríguez-Estrada C. Fluorometric determinations of NADH2 levels in dorsal root ganglion following peripheral nerve stimulation. Brain Res. 1967 Oct;6(2):217–227. doi: 10.1016/0006-8993(67)90192-8. [DOI] [PubMed] [Google Scholar]
  15. WU R., RACKER E. Regulatory mechanisms in carbohydrate metabolism. IV. Pasteur effect and Crabtree effect in ascites tumor cells. J Biol Chem. 1959 May;234(5):1036–1041. [PubMed] [Google Scholar]
  16. WU R. Regulatory mechanisms in carbohydrate metabolism. V. Limiting factors of glycolysis in HeLa cells. J Biol Chem. 1959 Nov;234:2806–2810. [PubMed] [Google Scholar]
  17. Williamson J. R., Cheung W. Y., Coles H. S., Herczeg B. E. Glycolytic control mechanisms. IV. Kinetics of glycolytic intermediate changes during electrical discharge and recovery in the main organ of Electrophorus electricus. J Biol Chem. 1967 Nov 10;242(21):5112–5118. [PubMed] [Google Scholar]
  18. Williamson J. R., Herczeg B. E., Coles H. S., Cheung W. Y. Glycolytic control mechanisms. V. Kinetics of high energy phosphate intermediate changes during electrical discharge and recovery in the main organ of Electrophorus electricus. J Biol Chem. 1967 Nov 10;242(21):5119–5124. [PubMed] [Google Scholar]
  19. den Hertog A., Greengard P., Ritchie J. M. On the metabolic basis of nervous activity. J Physiol. 1969 Oct;204(3):511–521. doi: 10.1113/jphysiol.1969.sp008928. [DOI] [PMC free article] [PubMed] [Google Scholar]

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