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. 1971 Jan;212(2):337–351. doi: 10.1113/jphysiol.1971.sp009328

Anion interaction at the inhibitory post-synaptic membrane of the crayfish neuromuscular junction

A Takeuchi, Noriko Takeuchi
PMCID: PMC1395668  PMID: 5548011

Abstract

1. The membrane potential and the membrane conductance of the crayfish muscle fibre in solutions containing various anions were measured with intracellular micro-electrodes.

2. When Cl- in the solution was replaced by Br-, NO3-, I- or CNS-, the addition of γ-amino butyric acid (GABA) produced a transient hyperpolarization of the membrane.

3. The reversal potential of the inhibitory junctional potentials (i.j.p.s) was at a slightly depolarized level relative to the resting potential in the normal Cl- solution. When Cl- in the bathing solution was replaced by foreign anions, the reversal potential shifted towards the hyperpolarized level. The hyperpolarization was in the order CNS- > I- > NO3- > Br-.

4. When a part of Cl- in the bathing solution was substituted by Br-, the inhibitory membrane conductance activated by GABA was increased as the concentration of Br- increased. The inhibitory membrane conductance decreased when one quarter or a half of Cl- was replaced by CNS-, NO3- or I-, but it increased again in higher concentrations of these anions.

5. Ca2+, Mg2+ and Ba2+ showed no appreciable effect on the inhibitory membrane conductance activated by GABA, while they decreased the conductance of the resting muscle membrane.

6. It was suggested that, at the activated inhibitory membrane, there is an interaction between anions and the permeability of Cl- is decreased by the presence of foreign anions, such as CNS-, I- and NO3-. The present results support the idea that the activated inhibitory membrane is charged positively and anions penetrate the membrane interacting with the charge sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. Internal chloride concentration and chloride efflux of frog muscle. J Physiol. 1961 May;156:623–632. doi: 10.1113/jphysiol.1961.sp006698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOISTEL J., FATT P. Membrane permeability change during inhibitory transmitter action in crustacean muscle. J Physiol. 1958 Nov 10;144(1):176–191. doi: 10.1113/jphysiol.1958.sp006094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURKE W., GINSBORG B. L. The action of the neuromuscular transmitter on the slow fibre membrane. J Physiol. 1956 Jun 28;132(3):599–610. doi: 10.1113/jphysiol.1956.sp005552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONTI F., EISENMAN G. THE NON-STEADY STATE MEMBRANE POTENTIAL OF ION EXCHANGERS WITH FIXED SITES. Biophys J. 1965 Mar;5:247–256. doi: 10.1016/s0006-3495(65)86714-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUDEL J., KUFFLER S. W. Presynaptic inhibition at the crayfish neuromuscular junction. J Physiol. 1961 Mar;155:543–562. doi: 10.1113/jphysiol.1961.sp006646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EDWARDS C., HARRIS E. J., NISHIE K. The exchange of frog muscle Na+ and K+ in the presence of the anions Br-, NO3-, I- and CNS-. J Physiol. 1957 Mar 11;135(3):560–566. doi: 10.1113/jphysiol.1957.sp005730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eisenman G., Sandblom J. P., Walker J. L., Jr Membrane structure and ion permeation. Study of ion exchange membrane structure and function is relevant to analysis of biological ion permeation. Science. 1967 Feb 24;155(3765):965–974. doi: 10.1126/science.155.3765.965. [DOI] [PubMed] [Google Scholar]
  8. FATT P., KATZ B. The effect of inhibitory nerve impulses on a crustacean muscle fibre. J Physiol. 1953 Aug;121(2):374–389. doi: 10.1113/jphysiol.1953.sp004952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GRUNDFEST H., REUBEN J. P., RICKLES W. H., Jr The electrophysiology and pharmacology of lobster neuromuscular synapses. J Gen Physiol. 1959 Jul 20;42(6):1301–1323. doi: 10.1085/jgp.42.6.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HARRIS E. J. Anion interaction in frog muscle. J Physiol. 1958 Apr 30;141(2):351–365. doi: 10.1113/jphysiol.1958.sp005979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOROWICZ P. THE EFFECTS OF ANIONS ON EXCITABLE CELLS. Pharmacol Rev. 1964 Jun;16:193–221. [PubMed] [Google Scholar]
  14. HUTTER O. F., PADSHA S. M. Effect of nitrate and other anions on the membrane resistance of frog skeletal muscle. J Physiol. 1959 Apr 23;146(1):117–132. doi: 10.1113/jphysiol.1959.sp006182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hutter O. F., Warner A. E. Action of some foreign cations and anions on the chloride permeability of frog muscle. J Physiol. 1967 Apr;189(3):445–460. doi: 10.1113/jphysiol.1967.sp008178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hutter O. F., Warner A. E. The pH sensitivity of the chloride conductance of frog skeletal muscle. J Physiol. 1967 Apr;189(3):403–425. doi: 10.1113/jphysiol.1967.sp008176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moore L. E. Anion permeability of frog skeletal muscle. J Gen Physiol. 1969 Jul;54(1):33–52. doi: 10.1085/jgp.54.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Motokizawa F., Reuben J. P., Grundfest H. Ionic permeability of the inhibitory postsynaptic membrane of lobster muscle fibers. J Gen Physiol. 1969 Oct;54(4):437–461. doi: 10.1085/jgp.54.4.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TAKEUCHI N. Effects of calcium on the conductance change of the end-plate membrane during the action of transmitter. J Physiol. 1963 Jun;167:141–155. doi: 10.1113/jphysiol.1963.sp007137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WASHIO H., MASHIMA H. EFFECTS OF SOME ANIONS AND CATIONS ON THE MEMBRANE RESISTANCE AND TWITCH TENSION OF FROG MUSCLE FIBRE. Jpn J Physiol. 1963 Dec 15;13:617–629. doi: 10.2170/jjphysiol.13.617. [DOI] [PubMed] [Google Scholar]

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