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. 1971 Jan;212(1):259–275. doi: 10.1113/jphysiol.1971.sp009321

Changes in light scattering associated with the action potential in crab nerves

L B Cohen, R D Keynes
PMCID: PMC1395691  PMID: 5545182

Abstract

1. Changes in light scattering from stimulated crab leg nerves were measured in an effort to study changes in structure that occur during the action potential.

2. The peak changes in scattering after individual stimuli were 10-4-10-5 of the resting scattering; signal averaging techniques were used to increase the signal-to-noise ratio.

3. During the compound action potential there was a transient increase in the 90° scattering which reached a peak about 20 msec after the stimulus. This increase was probably not due to a change in axon volume. Its time course was somewhat slower than that of birefringence changes measured at the same time.

4. In normal sea water the transient increase was followed by a longer lasting (persistent) decrease in scattering. This persistent decrease could be converted to an increase by reducing the tonicity of the sea water or by raising the external refractive index. This scattering change may represent a swelling of the axon resulting from the ionic exchanges that occur during the action potential.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABBOTT B. C., HILL A. V., HOWARTH J. V. The positive and negative heat production associated with a nerve impulse. Proc R Soc Lond B Biol Sci. 1958 Feb 18;148(931):149–187. doi: 10.1098/rspb.1958.0012. [DOI] [PubMed] [Google Scholar]
  2. BRYANT S. H., TOBIAS J. M. Changes in light scattering accompanying activity in nerve. J Cell Physiol. 1952 Oct;40(2):199–219. doi: 10.1002/jcp.1030400204. [DOI] [PubMed] [Google Scholar]
  3. BRYANT S. H., TOBIAS J. M. Optical and mechanical concomitants of activity in Carcinus nerve. I. Effect of sodium azide on the optical response. II. Shortening of the nerve with activity. J Cell Physiol. 1955 Aug;46(1):71–95. doi: 10.1002/jcp.1030460105. [DOI] [PubMed] [Google Scholar]
  4. Baker P. F., Blaustein M. P. Sodium-dependent uptake of calcium by crab nerve. Biochim Biophys Acta. 1968 Jan 3;150(1):167–170. doi: 10.1016/0005-2736(68)90023-0. [DOI] [PubMed] [Google Scholar]
  5. Baker P. F. Phosphorus metabolism of intact crab nerve and its relation to the active transport of ions. J Physiol. 1965 Sep;180(2):383–423. doi: 10.1113/jphysiol.1965.sp007709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CALDWELL P. C., KEYNES R. D. The permeability of the squid giant axon to radioactive potassium and chloride ions. J Physiol. 1960 Nov;154:177–189. doi: 10.1113/jphysiol.1960.sp006572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen L. B., Hille B., Keynes R. D. Light scattering and birefringence changes during activity in the electric organ of electrophorus electricus. J Physiol. 1969 Aug;203(2):489–509. doi: 10.1113/jphysiol.1969.sp008876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cohen L. B., Keynes R. D. Evidence for structural changes during the action potential in nerves from the walking legs of Maia squinado. J Physiol. 1968 Feb;194(2):85–6P. [PubMed] [Google Scholar]
  9. Cohen L. B., Keynes R. D., Hille B. Light scattering and birefringence changes during nerve activity. Nature. 1968 May 4;218(5140):438–441. doi: 10.1038/218438a0. [DOI] [PubMed] [Google Scholar]
  10. Furusawa K. The depolarization of crustacean nerve by stimulation or oxygen want. J Physiol. 1929 Jul 25;67(4):325–342. doi: 10.1113/jphysiol.1929.sp002573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HILL D. K. The effect of stimulation on the opacity of a crustacean nerve trunk and its relation to fibre diameter. J Physiol. 1950 Oct 16;111(3-4):283–303. doi: 10.1113/jphysiol.1950.sp004480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HILL D. K. The volume change resulting from stimulation of a giant nerve fibre. J Physiol. 1950 Oct 16;111(3-4):304–327. doi: 10.1113/jphysiol.1950.sp004481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hill D. K., Keynes R. D. Opacity changes in stimulated nerve. J Physiol. 1949 May 15;108(3):278–281. [PMC free article] [PubMed] [Google Scholar]
  14. KEYNES R. D. The ionic movements during nervous activity. J Physiol. 1951 Jun;114(1-2):119–150. doi: 10.1113/jphysiol.1951.sp004608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SHAW S. N., TOBIAS J. M. On the optical change associated with activity in frog nerve. J Cell Physiol. 1955 Aug;46(1):53–70. doi: 10.1002/jcp.1030460104. [DOI] [PubMed] [Google Scholar]
  16. TOBIAS J. M. Some optically detectable consequences of activity in nerve. Cold Spring Harb Symp Quant Biol. 1952;17:15–25. doi: 10.1101/sqb.1952.017.01.004. [DOI] [PubMed] [Google Scholar]
  17. Tasaki I., Watanabe A., Sandlin R., Carnay L. Changes in fluorescence, turbidity, and birefringence associated with nerve excitation. Proc Natl Acad Sci U S A. 1968 Nov;61(3):883–888. doi: 10.1073/pnas.61.3.883. [DOI] [PMC free article] [PubMed] [Google Scholar]

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