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. 1971 Jan;212(1):85–99. doi: 10.1113/jphysiol.1971.sp009311

Determination of extracellular space and intracellular electrolytes in rat liver in vivo*

J A Williams, D M Woodbury
PMCID: PMC1395694  PMID: 5545186

Abstract

1. Uptake of a number of isotopically labelled extracellular tracers by rat liver in vivo was studied.

2. A residual intravascular space of 3·0-3·5% following exsanguination was measured by use of [51Cr]red cells. This space was significantly greater than that of skeletal muscle and was also greater in nephrectomized as compared to intact rats.

3. [131I]albumin and polyvinylpyrolidine (PVP) were found to distribute initially in a space of 6-7% and this was followed by a delayed rise that was greater for PVP. The extravascular portion of this space is termed the large-molecule accessible space and is believed to correspond to the histological space of Disse.

4. [14C]sucrose and inulin initially distribute in a space of 10-12% followed by a continued late increase. This initial space is termed the small molecule accessible space and is thought to include most of interstitial space.

5. 36Cl- and stable Na+ and Cl- spaces were constant at 19-21% and can be used to put an upper limit on extracellular space.

6. Operationally, the 1 hr inulin space in the nephrectomized rat was felt to be the best indicator of extracellular space.

7. Total electrolytes were measured and intracellular values of 22, 165 and 22 m-mole/l. cell water for Na+, K+ and Cl-, respectively, were calculated.

8. By use of [14C]DMO, an intracellular pH of 7·25 and an intracellular HCO3- of 13 m-mole/l. cell water were calculated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBER-RILEY G. MEASUREMENT OF CAPACITY OF BILIARY TREE IN RATS. Am J Physiol. 1963 Dec;205:1122–1126. doi: 10.1152/ajplegacy.1963.205.6.1122. [DOI] [PubMed] [Google Scholar]
  2. BRAUER R. W., HOLLOWAY R. J., LEONG G. F. Changes in liver function and structure due to experimental passive congestion under controlled hepatic vein pressures. Am J Physiol. 1959 Sep;197:681–692. doi: 10.1152/ajplegacy.1959.197.3.681. [DOI] [PubMed] [Google Scholar]
  3. BRAUER R. W. Liver circulation and function. Physiol Rev. 1963 Jan;43:115–213. doi: 10.1152/physrev.1963.43.1.115. [DOI] [PubMed] [Google Scholar]
  4. CAHILL G. F., Jr, ASHMORE J., EARLE A. S., ZOTTU S. Glucose penetration into liver. Am J Physiol. 1958 Mar;192(3):491–496. doi: 10.1152/ajplegacy.1958.192.3.491. [DOI] [PubMed] [Google Scholar]
  5. CHENDEROVITCH J., PHOCAS E., TROUPEL S., RENAULT H., CAROLI J. [The transfer of large molecules from the blood into the bile and hepatic lymph in the guinea-pig. Study on polyvinylpyrrolidone-I-131]. Rev Fr Etud Clin Biol. 1961 May;6:470–472. [PubMed] [Google Scholar]
  6. COOK D. L., LAWLER C. A., CALVIN L. D., GREEN D. M. Mechanisms of bile formation. Am J Physiol. 1952 Oct;171(1):62–74. doi: 10.1152/ajplegacy.1952.171.1.62. [DOI] [PubMed] [Google Scholar]
  7. Chow S. Y., Jee W. S., Taylor G. N., Woodbury D. M. Radioautographic studies of inulin, sulfate and chloride in rat and guinea pig thyroid glands. Endocrinology. 1965 Nov;77(5):818–824. doi: 10.1210/endo-77-5-818. [DOI] [PubMed] [Google Scholar]
  8. Craig A. B., Jr Glucose, lactate and potassium metabolism in the isolated perfused rat liver. Proc Soc Exp Biol Med. 1966 Jan;121(1):281–286. doi: 10.3181/00379727-121-30759. [DOI] [PubMed] [Google Scholar]
  9. DULCE H. J., GUENTHER T. H. [The controlling of cellular electrolyte and water content by hormones]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1960;238:368–390. [PubMed] [Google Scholar]
  10. ELLIOTT K. A., PAPPIUS H. M. Water distribution in incubated slices of brain and other tissues. Can J Biochem Physiol. 1956 Sep;34(5):1007–1022. [PubMed] [Google Scholar]
  11. EVERETT N. B., SIMMONS B., LASHER E. P. Distribution of blood (Fe 59) and plasma (I 131) volumes of rats determined by liquid nitrogen freezing. Circ Res. 1956 Jul;4(4):419–424. doi: 10.1161/01.res.4.4.419. [DOI] [PubMed] [Google Scholar]
  12. GORESKY C. A. A linear method for determining liver sinusoidal and extravascular volumes. Am J Physiol. 1963 Apr;204:626–640. doi: 10.1152/ajplegacy.1963.204.4.626. [DOI] [PubMed] [Google Scholar]
  13. HECKMANN K. D., PARSONS D. S. Changes in the water and electrolyte content of rat-liver slices in vitro. Biochim Biophys Acta. 1959 Nov;36:203–213. doi: 10.1016/0006-3002(59)90085-x. [DOI] [PubMed] [Google Scholar]
  14. HENDLER R. W. PROCEDURE FOR SIMULTANEOUS ASSAY OF TWO BETA-EMITTING ISOTOPES WITH THE LIQUID SCINTILLATION COUNTING TECHNIQUE. Anal Biochem. 1964 Jan;7:110–120. doi: 10.1016/0003-2697(64)90125-3. [DOI] [PubMed] [Google Scholar]
  15. Hulme B., Dykes P. W., Appleyard I., Arkell D. W. Retention and storage sites of radioactive polyvinylpyrrolidone. J Nucl Med. 1968 Jul;9(7):389–393. [PubMed] [Google Scholar]
  16. IRVINE R. O., SAUNDERS S. J., MILNE M. D., CRAWFORD M. A. Gradients of potassium and hydrogen ion in potassiumdeficient voluntary muscle. Clin Sci. 1961 Feb;20:1–18. [PubMed] [Google Scholar]
  17. LEAF A. On the mechanism of fluid exchange of tissues in vitro. Biochem J. 1956 Feb;62(2):241–248. doi: 10.1042/bj0620241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOSERT W., SENFT C., SENFT G. EXTRARENALE WIRKUNGEN DES ALDOSTERONS UND DER SPIROLACTONE. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 Jul 20;248:450–463. doi: 10.1007/BF00246891. [DOI] [PubMed] [Google Scholar]
  19. Lode H., Senft G., Losert W. Der Einfluss eines Kaliummangels auf die Verteilung von Lithiumionen zwischen dem intra- und extracellulären Raum. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1967;258(5):418–429. [PubMed] [Google Scholar]
  20. PARSONS D. S., VAN ROSSUM G. D. On the determination of the extracellular water compartment in swollen slices of rat liver. Biochim Biophys Acta. 1962 Mar 12;57:495–508. doi: 10.1016/0006-3002(62)91157-5. [DOI] [PubMed] [Google Scholar]
  21. REED D. J., WOODBURY D. M. KINETICS OF MOVEMENT OF IODIDE, SUCROSE, INULIN AND RADIO-IODINATED SERUM ALBUMIN IN THE CENTRAL NERVOUS SYSTEM AND CEREBROSPINAL FLUID OF THE RAT. J Physiol. 1963 Dec;169:816–850. doi: 10.1113/jphysiol.1963.sp007298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SCHANKER L. S., HOGBEN C. A. Biliary excretion of inulin, sucrose, and mannitol: analysis of bile formation. Am J Physiol. 1961 May;200:1087–1090. doi: 10.1152/ajplegacy.1961.200.5.1087. [DOI] [PubMed] [Google Scholar]
  23. STERLING K., GRAY S. J. Determination of the circulating red cell volume in man by radioactive chromium. J Clin Invest. 1950 Dec;29(12):1614–1619. doi: 10.1172/JCI102404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Senft G., Losert W., Sitt R., McEvoy J., Kaess H. Vergleichende Untersuchungen über den Einfluss von 6-Aminonicotinamid und 2,4-Dinitrophenol auf den Natium- und Kaliumtransport erschiedener Gewebe. Naunyn Schmiedebergs Arch Pharmakol Exp Pathol. 1966;255(4):388–397. [PubMed] [Google Scholar]
  25. Struyvenberg A., Morrison R. B., Relman A. S. Acid-base behavior of separated canine renal tubule cells. Am J Physiol. 1968 May;214(5):1155–1162. doi: 10.1152/ajplegacy.1968.214.5.1155. [DOI] [PubMed] [Google Scholar]
  26. WADDELL W. J., BUTLER T. C. Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog. J Clin Invest. 1959 May;38(5):720–729. doi: 10.1172/JCI103852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WHITE H. L., ROLF D. Whole body and tissue inulin and sucrose spaces in the rat. Am J Physiol. 1957 Jan;188(1):151–155. doi: 10.1152/ajplegacy.1956.188.1.151. [DOI] [PubMed] [Google Scholar]
  28. WOODBURY D. M. Extrarenal effects of desoxycorticosterone, adrenocortical extract and adrenocorticotrophic hormone on plasma and tissue electrolytes in fed and fasted rats. Am J Physiol. 1953 Jul;174(1):1–19. doi: 10.1152/ajplegacy.1953.174.1.1. [DOI] [PubMed] [Google Scholar]
  29. Wheeler H. O., Ross E. D., Bradley S. E. Canalicular bile production in dogs. Am J Physiol. 1968 Apr;214(4):866–874. doi: 10.1152/ajplegacy.1968.214.4.866. [DOI] [PubMed] [Google Scholar]
  30. Williams J. A., Withrow C. D., Woodbury D. M. Effects of nephrectomy and KC1 on transmembrane potentials, intracellular electrolytes, and cell pH of rat muscle and liver in vivo. J Physiol. 1971 Jan;212(1):117–128. doi: 10.1113/jphysiol.1971.sp009313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Williams J. A., Withrow C. D., Woodbury D. M. Effects of ouabain and diphenylhydantoin on transmembrane potentials, intracellular electrolytes, and cell pH of rat muscle and liver in vivo. J Physiol. 1971 Jan;212(1):101–115. doi: 10.1113/jphysiol.1971.sp009312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zieve P. D., Haghshenass M., Krevans J. R. Intracellular pH of the human lymphocyte. Am J Physiol. 1967 May;212(5):1099–1102. doi: 10.1152/ajplegacy.1967.212.5.1099. [DOI] [PubMed] [Google Scholar]

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