Abstract
1. Sacs of everted mid-small intestine of the hamster have been used to study the effect of amino acids on sugar absorption.
2. The sugars employed were D-glucose, D-galactose, 3-O-methyl-D-glucose, D-fucose, L-glucose, α-glucoheptose, L-fucose, D-mannose and L-sorbose. The amino acids were L- and D-histidine, L- and D-methionine, L- and D-alanine, L- and D-valine, L- and D-glutamic acid, L-leucine, L-proline, L-ornithine and L-aspartic acid.
3. Actively absorbed amino acids considerably inhibit the transport of actively absorbed sugars. The results give support for the view that D-histidine and L-glucose are actively transferred. Passively absorbed amino acids and sugars are not involved.
4. As L-glutamic and L-aspartic acids in the mucosal fluid have no inhibitory effect on D-glucose absorption, although mucosal fluid L-alanine is quite potent, the step at which the latter exerts its inhibitory action must be before that at which the intracellular transamination of L-glutamic and L-aspartic acids occurs. It would seem likely, therefore, that L-alanine interferes with the process by which epithelial cells capture and concentrate sugars at the luminal border.
5. More than one active transfer system may exist for D-glucose.
6. The influence of actively absorbed L-amino acids on D-glucose active transport seems to be in some way related to the efficiency with which the amino acids are themselves concentrated.
7. Inhibition of D-glucose active absorption by an amino acid may be a simple test of an amino acid's participation in an active transport system.
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CSAKY T. Z., FERNALD G. W. Absorption of 3-methylglucose from the intestine of the frog, Rana pipiens. Am J Physiol. 1960 Feb;198:445–448. doi: 10.1152/ajplegacy.1960.198.2.445. [DOI] [PubMed] [Google Scholar]
- FINCH L. R., HIRD F. J. The uptake of amino acids by isolated segments of rat intestine. II. A survey of affinity for uptake from rates of uptake and competition for uptake. Biochim Biophys Acta. 1960 Sep 23;43:278–287. doi: 10.1016/0006-3002(60)90438-8. [DOI] [PubMed] [Google Scholar]
- JERVIS E. L., SMYTH D. H. Competition between enantiomorphs of amino acids during intestinal absorption. J Physiol. 1959 Jan 28;145(1):57–65. doi: 10.1113/jphysiol.1959.sp006126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JERVIS E. L., SMYTH D. H. The active transfer of D-methionine by the rat intestine in vitro. J Physiol. 1960 Apr;151:51–58. [PMC free article] [PubMed] [Google Scholar]
- LARSEN P. R., ROSS J. E., TAPLEY D. F. TRANSPORT OF NEUTRAL, DIBASIC AND N-METHYL-SUBSTITUTED AMINO ACIDS BY RAT INTESTINE. Biochim Biophys Acta. 1964 Nov 29;88:570–577. doi: 10.1016/0926-6577(64)90100-7. [DOI] [PubMed] [Google Scholar]
- LIN E. C., HAGIHIRA H., WILSON T. H. Specificity of the transport system for neutral amino acids in the hamster intestine. Am J Physiol. 1962 May;202:919–925. doi: 10.1152/ajplegacy.1962.202.5.919. [DOI] [PubMed] [Google Scholar]
- MATTHEWS D. M., LASTER L. KINETICS OF INTESTINAL ACTIVE TRANSPORT OF FIVE NEUTRAL AMINO ACIDS. Am J Physiol. 1965 Apr;208:593–600. doi: 10.1152/ajplegacy.1965.208.4.593. [DOI] [PubMed] [Google Scholar]
- MATTHEWS D. M., WISEMAN G. Transamination by the small intestine of the rat. J Physiol. 1953 Jun 29;120(4):55P–55P. [PubMed] [Google Scholar]
- NEAME K. D., WISEMAN G. The alanine and oxo acid concentrations in mesenteric blood during the absorption of L-glutamic acid by the small intestine of the dog, cat and rabbit in vivo. J Physiol. 1958 Jan 23;140(1):148–155. doi: 10.1113/jphysiol.1958.sp005923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEAME K. D., WISEMAN G. The transamination of glutamic and aspartic acids during absorption by the small intestine of the dog in vivo. J Physiol. 1957 Feb 15;135(2):442–450. doi: 10.1113/jphysiol.1957.sp005722. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RANDALL H. G., EVERED D. F. AMINO ACID UPTAKE BY LIVING CELLS. I. AMINO GROUP REQUIREMENT BY SMALL INTESTINE OF THE RAT IN VITRO. Biochim Biophys Acta. 1964 Oct 9;93:98–105. doi: 10.1016/0304-4165(64)90264-8. [DOI] [PubMed] [Google Scholar]
- WILSON T. H., WISEMAN G. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J Physiol. 1954 Jan;123(1):116–125. doi: 10.1113/jphysiol.1954.sp005036. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WISEMAN G. Preferential transference of amino-acids from amino-acid mixtures by sacs of everted small intestine of the golden hamster (Mesocricetus auratus). J Physiol. 1955 Feb 28;127(2):414–422. doi: 10.1113/jphysiol.1955.sp005267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WISEMAN G. Sac of everted intestine technic for study of intestinal absorption in vitro. Methods Med Res. 1961;9:287–292. [PubMed] [Google Scholar]