Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1966 Oct;186(3):619–631. doi: 10.1113/jphysiol.1966.sp008059

Regulation of amino acid transport across intestines of goldfish acclimatized to different environmental temperatures

T B Mepham, M W Smith
PMCID: PMC1395926  PMID: 5972157

Abstract

1. Serosal transfers of valine and threonine were measured using everted sacs of anterior intestine taken from goldfish acclimatized to different temperatures.

2. Both valine and threonine were actively transported at incubation temperatures equal to or greater than the previous environmental temperature of the fish. There was also a positive serosal transfer of valine, but not threonine, at incubation temperatures below the previous environmental temperature of the fish.

3. The mean stable transmural potentials and amino-acid-evoked potentials depended both on the temperature to which the fish had been acclimatized and on the temperature at which the sacs were incubated.

4. There was a linear relation between the transmural potential and the serosal transfer of amino acid, one additional μmole of valine or threonine being transferred/2 hr incubation period for each 3 mV rise in potential. There was a less obvious correlation between the amino-acid-evoked potential and on serosal transfer of amino acid.

5. Acclimatization of the goldfish intestine from 8 to 25° C, assessed by changes occurring in the transmural potential and serosal transfer of amino acids, tended to stabilize both parameters, but the compensation in each case was only partial.

6. It is possible that the imbalance in transfer of valine-like and threonine-like amino acids, seen at incubation temperatures below the previous acclimatization temperature of the fish, has a special function in initiating the process of acclimatization to the new environmental temperature.

Full text

PDF
619

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AGAR W. T., HIRD F. J., SIDHU G. S. The uptake of amino acids by the intestine. Biochim Biophys Acta. 1954 May;14(1):80–84. doi: 10.1016/0006-3002(54)90134-1. [DOI] [PubMed] [Google Scholar]
  2. AKEDO H., CHRISTENSEN H. N. Transfer of amino acids across the intestine: a new model amino acid. J Biol Chem. 1962 Jan;237:113–117. [PubMed] [Google Scholar]
  3. ASANO T. METABOLIC DISTURBANCES AND SHORT-CIRCUIT CURRENT ACROSS INTESTINAL WALL OF RAT. Am J Physiol. 1964 Aug;207:415–422. doi: 10.1152/ajplegacy.1964.207.2.415. [DOI] [PubMed] [Google Scholar]
  4. Barry R. J., Smyth D. H., Wright E. M. Short-circuit current and solute transfer by rat jejunum. J Physiol. 1965 Nov;181(2):410–431. doi: 10.1113/jphysiol.1965.sp007770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COHEN G. N. Synthèse de protéines anormales chez Escherichia coli K 12 cultive en présence de L-valine. Ann Inst Pasteur (Paris) 1958 Jan;94(1):15–30. [PubMed] [Google Scholar]
  6. Curran P. F. Ion transport in intestine and its coupling to other transport processes. Fed Proc. 1965 Sep-Oct;24(5):993–999. [PubMed] [Google Scholar]
  7. Gilles-Baillien M., Schoffeniels E. Site of action of L-alanine and D-glucose on the potential difference across the intestine. Arch Int Physiol Biochim. 1965 Mar;73(2):355–357. doi: 10.3109/13813456509084257. [DOI] [PubMed] [Google Scholar]
  8. Mepham T. B., Smith M. W. Amino acid transport in the goldfish intestine. J Physiol. 1966 Jun;184(3):673–684. doi: 10.1113/jphysiol.1966.sp007940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  10. OXENDER D. L., CHRISTENSEN H. N. Evidence for two types of mediation of neutral and amino-acid transport in Ehrlich cells. Nature. 1963 Feb 23;197:765–767. doi: 10.1038/197765a0. [DOI] [PubMed] [Google Scholar]
  11. Rosenberg I. H., Coleman A. L., Rosenberg L. E. The role of sodium ion in the transport of amino acids by the intestine. Biochim Biophys Acta. 1965 May 25;102(1):161–171. doi: 10.1016/0926-6585(65)90210-4. [DOI] [PubMed] [Google Scholar]
  12. SCHULTZ S. G., ZALUSKY R. INTERACTIONS BETWEEN ACTIVE SODIUM TRANSPORT AND ACTIVE AMINO-ACID TRANSPORT IN ISOLATED RABBIT ILEUM. Nature. 1965 Jan 16;205:292–294. doi: 10.1038/205292a0. [DOI] [PubMed] [Google Scholar]
  13. SCHULTZ S. G., ZALUSKY R. ION TRANSPORT IN ISOLATED RABBIT ILEUM. I. SHORT-CIRCUIT CURRENT AND NA FLUXES. J Gen Physiol. 1964 Jan;47:567–584. doi: 10.1085/jgp.47.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STEIN W. H., MOORE S. The free amino acids of human blood plasma. J Biol Chem. 1954 Dec;211(2):915–926. [PubMed] [Google Scholar]
  15. Smith M. W. Influence of temperature acclimatization on sodium--glucose interactions in the goldfish intestine. J Physiol. 1966 Feb;182(3):574–590. doi: 10.1113/jphysiol.1966.sp007838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith M. W. Puromycin inhibition of changes in glucose-evoked potential recorded in goldfish intestine after periods of temperature acclimatization. Experientia. 1966 Apr 15;22(4):252–253. doi: 10.1007/BF01900943. [DOI] [PubMed] [Google Scholar]
  17. Smith M. W. Sodium-glucose interactions in the goldfish intestine. J Physiol. 1966 Feb;182(3):559–573. doi: 10.1113/jphysiol.1966.sp007837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WISEMAN G. Active transport of amino acids by sacs of everted small intestine of the golden hamster (Mesocricetus auratus). J Physiol. 1956 Sep 27;133(3):626–630. doi: 10.1113/jphysiol.1956.sp005614. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES