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. 1967 Jan;188(2):177–190. doi: 10.1113/jphysiol.1967.sp008132

Active sodium transport by the colon of Bufo marinus: stimulation by aldosterone and antidiuretic hormone

G Cofré, J Crabbé
PMCID: PMC1396004  PMID: 16992237

Abstract

1. The isolated colon of Bufo marinus transports sodium actively from the mucosal (lumen) to the serosal side, and this transport is expressed quantitatively by the short-circuit current.

2. Upon dilution of sodium in Ringer solution on the mucosal side of the preparation, short-circuit current remained a fair expression of sodium transport from mucosa to serosa.

3. In view of this, the relation between short-circuit current and dilution of sodium of the luminal side was examined. This relation was curvilinear, which suggests the intervention of a saturable step in the transfer of sodium from lumen to serosal surface of colon.

4. The relation between short-circuit current on the one hand, and the amount of sodium drawn from the luminal side and recovered in the membrane (`active sodium transport pool') on the other hand, appeared (almost) linear instead. This is meant to indicate that the `pump' operates far from capacity. Hence, the observed saturation of sodium transport, when concentration of sodium on the mucosal side was increased, probably occurs at the mucosal border of the preparation.

5. After treatment with aldosterone, the `active sodium transport pool' and short-circuit current increased to the same extent, from which it is inferred that the hormone merely allows sodium easier access to the `pump' which would react in proportion. Consequently, no direct influence of aldosterone on the `pump' proper need be postulated.

6. Upon exposure of the colon to antidiuretic hormone, there were (modest) increases of short-circuit current and of osmotic water flow across the wall of the organ.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASANO T. METABOLIC DISTURBANCES AND SHORT-CIRCUIT CURRENT ACROSS INTESTINAL WALL OF RAT. Am J Physiol. 1964 Aug;207:415–422. doi: 10.1152/ajplegacy.1964.207.2.415. [DOI] [PubMed] [Google Scholar]
  2. CEREIJIDO M., HERRERA F. C., FLANIGAN W. J., CURRAN P. F. THE INFLUENCE OF NA CONCENTRATION ON NA TRANSPORT ACROSS FROG SKIN. J Gen Physiol. 1964 May;47:879–893. doi: 10.1085/jgp.47.5.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COOPERSTEIN I. L., HOGBEN C. A. Ionic transfer across the isolated frog large intestine. J Gen Physiol. 1959 Jan 20;42(3):461–473. doi: 10.1085/jgp.42.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CRABBE J. DECREASED EFFECTIVENESS OF ALDOSTERONE ON ACTIVE SODIUM TRANSPORT BY THE ISOLATED TOAD BLADDER IN THE PRESENCE OF OTHER STEROIDS. Acta Endocrinol (Copenh) 1964 Nov;47:419–432. doi: 10.1530/acta.0.0470419. [DOI] [PubMed] [Google Scholar]
  5. CRABBE J. STIMULATION BY ALDOSTERONE OF ACTIVE SODIUM TRANSPORT ACROSS THE ISOLATED VENTRAL SKIN OF AMPHIBIA. Endocrinology. 1964 Nov;75:809–811. doi: 10.1210/endo-75-5-809. [DOI] [PubMed] [Google Scholar]
  6. CRABBE J. Stimulation of active sodium transport by the isolated toad bladder with aldosterone in vitro. J Clin Invest. 1961 Nov;40:2103–2110. doi: 10.1172/JCI104436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CURRAN P. F., SOLOMON A. K. Ion and water fluxes in the ileum of rats. J Gen Physiol. 1957 Sep 20;41(1):143–168. doi: 10.1085/jgp.41.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cofré G., Crabbé J. Stimulation by aldosterone of active sodium transport by the isolated colon of the toad, Bufo marinus. Nature. 1965 Sep 18;207(5003):1299–1300. doi: 10.1038/2071299a0. [DOI] [PubMed] [Google Scholar]
  9. Crabbé J., De Weer P. Action of aldosterone and vasopressin on the active transport of sodium by the isolated toad bladder. J Physiol. 1965 Oct;180(3):560–568. doi: 10.1113/jphysiol.1965.sp007717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FRAZIER H. S., DEMPSEY E. F., LEAF A. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin. J Gen Physiol. 1962 Jan;45:529–543. doi: 10.1085/jgp.45.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FRAZIER H. S. The electrical potential profile of the isolated toad bladder. J Gen Physiol. 1962 Jan;45:515–528. doi: 10.1085/jgp.45.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  13. LEAF A., ANDERSON J., PAGE L. B. Active sodium transport by the isolated toad bladder. J Gen Physiol. 1958 Mar 20;41(4):657–668. doi: 10.1085/jgp.41.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LEVITAN R., INGELFINGER F. J. EFFECT OF D-ALDOSTERONE ON SALT AND WATER ABSORPTION FROM THE INTACT HUMAN COLON. J Clin Invest. 1965 May;44:801–808. doi: 10.1172/JCI105192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MOLL H. C., KOCZOREK K. R. [On the influence of an aldosterone antagonist (SC-8109) on the absorption of sodium from the gastrointestinal tract of rats]. Klin Wochenschr. 1962 Aug 15;40:825–827. doi: 10.1007/BF01492710. [DOI] [PubMed] [Google Scholar]
  16. PORTER G. A., EDELMAN I. S. THE ACTION OF ALDOSTERONE AND RELATED CORTICOSTEROIDS ON SODIUM TRANSPORT ACROSS THE TOAD BLADDER. J Clin Invest. 1964 Apr;43:611–620. doi: 10.1172/JCI104946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROSS E. J., SPENCER A. G. Observations on cation exchange resins in the small and large intestines. Clin Sci. 1954 Nov;13(4):555–566. [PubMed] [Google Scholar]
  18. SCHULTZ S. G., ZALUSKY R. ION TRANSPORT IN ISOLATED RABBIT ILEUM. I. SHORT-CIRCUIT CURRENT AND NA FLUXES. J Gen Physiol. 1964 Jan;47:567–584. doi: 10.1085/jgp.47.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SHARP G. W., LEAF A. BIOLOGICAL ACTION OF ALDOSTERONE IN VITRO. Nature. 1964 Jun 20;202:1185–1188. doi: 10.1038/2021185a0. [DOI] [PubMed] [Google Scholar]
  20. URANGA J. Absorción de agua en el intestino del sapo. Rev Soc Argent Biol. 1958 Aug-Sep;34(5-6):161–164. [PubMed] [Google Scholar]
  21. USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]

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