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. 1967 Feb;188(3):387–402. doi: 10.1113/jphysiol.1967.sp008145

The role of α- and β-adrenergic receptors in some actions of catecholamines on intestinal smooth muscle

D H Jenkinson, I K M Morton
PMCID: PMC1396027  PMID: 6032206

Abstract

1. Experiments were carried out to determine the type of adrenergic receptor concerned in the action of noradrenaline in increasing the rate of exchange of potassium ions in the taenia of the guinea-pig caecum. The preparations were bathed in potassium-rich solutions in order to depolarize the muscle fibres and so eliminate changes in potassium flux secondary to alterations in membrane potential.

2. Noradrenaline was more effective than isoprenaline in increasing both the influx and efflux of 42K, suggesting that the α-receptors were involved. This action on potassium permeability was abolished by the α-blocking agent phentolamine (10-7 g/ml.), the β-blocking agent pronethalol (10-7 g/ml.) being without effect.

3. In contrast, isoprenaline was about 30 times more active than noradrenaline in inhibiting calcium contractures of the depolarized taenia, suggesting that here the β-receptors were concerned. This was confirmed by showing that this action of noradrenaline was effectively antagonized by pronethalol, again at 10-7 g/ml., but not by the same concentration of phentolamine.

4. Carbachol contractures in quiescent preparations bathed in Krebs solution at 17° C could be inhibited by both noradrenaline and isoprenaline. Noradrenaline was found to be more active under these conditions, and this action was antagonized by phentolamine (and piperoxane), but not by pronethalol, at the same concentrations as before.

5. These findings confirm that the α- and β-receptors are quite distinct in their actions. Nevertheless, under physiological conditions, both may contribute to the inhibitory actions of catecholamines on intestinal smooth muscle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AHLQUIST R. P., LEVY B. Andrenergic receptive mechanism of canine ileum. J Pharmacol Exp Ther. 1959 Oct;127:146–149. [PubMed] [Google Scholar]
  2. AHLQUIST R. P. The adrenotropic receptor-detector. Arch Int Pharmacodyn Ther. 1962 Sep 1;139:38–41. [PubMed] [Google Scholar]
  3. ALI H. I., ANTONIO A., HAUGAARD N. THE ACTION OF SYMPATHOMIMETIC AMINES AND ADRENERGIC BLOCKING AGENTS ON TISSUE PHOSPHORYLASE ACTIVITY. J Pharmacol Exp Ther. 1964 Aug;145:142–150. [PubMed] [Google Scholar]
  4. BLACK J. W., STEPHENSON J. S. Pharmacology of a new adrenergic beta-receptor-blocking compound (Nethalide). Lancet. 1962 Aug 18;2(7251):311–314. doi: 10.1016/s0140-6736(62)90103-4. [DOI] [PubMed] [Google Scholar]
  5. BUCKNELL A., WHITNEY B. A PRELIMINARY INVESTIGATION OF THE PHARMACOLOGY OF THE HUMAN ISOLATED TAENIA COLI PREPARATION. Br J Pharmacol Chemother. 1964 Aug;23:164–175. doi: 10.1111/j.1476-5381.1964.tb01576.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bennett M. R., Burnstock G., Holman M. Transmission from intramural inhibitory nerves to the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Feb;182(3):541–558. doi: 10.1113/jphysiol.1966.sp007836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Burnstock G., Campbell G., Rand M. J. The inhibitory innervation of the taenia of the guinea-pig caecum. J Physiol. 1966 Feb;182(3):504–526. doi: 10.1113/jphysiol.1966.sp007834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Burnstock G., Holman M. E. Effect of drugs on smooth muscle. Annu Rev Pharmacol. 1966;6:129–156. doi: 10.1146/annurev.pa.06.040166.001021. [DOI] [PubMed] [Google Scholar]
  9. DURBIN R. P., JENKINSON D. H. The calcium dependence of tension development in depolarized smooth muscle. J Physiol. 1961 Jun;157:90–96. doi: 10.1113/jphysiol.1961.sp006707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DURBIN R. P., JENKINSON D. H. The effect of carbachol on the permeability of depolarized smooth muscle to inorganic ions. J Physiol. 1961 Jun;157:74–89. doi: 10.1113/jphysiol.1961.sp006706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dale H. H. On some physiological actions of ergot. J Physiol. 1906 May 31;34(3):163–206. doi: 10.1113/jphysiol.1906.sp001148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. EDMAN K. A., SCHILD H. O. CALCIUM AND THE STIMULANT AND INHIBITORY EFFECTS OF ADRENALINE IN DEPOLARIZED SMOOTH MUSCLE. J Physiol. 1963 Nov;169:404–411. doi: 10.1113/jphysiol.1963.sp007265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. EDMAN K. A., SCHILD H. O. The need for calcium in the contractile responses induced by acetylcholine and potassium in the rat uterus. J Physiol. 1962 May;161:424–441. doi: 10.1113/jphysiol.1962.sp006897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. EVANS D. H., SCHILD H. O., THESLEFF S. Effects of drugs on depolarized plain muscle. J Physiol. 1958 Oct 31;143(3):474–485. doi: 10.1113/jphysiol.1958.sp006072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HORNBROOK K. R., BRODY T. M. PHOSPHORYLASE ACTIVITY IN RAT LIVER AND SKELETAL MUSCLE AFTER CATECHOLAMINES. Biochem Pharmacol. 1963 Dec;12:1407–1415. doi: 10.1016/0006-2952(63)90210-7. [DOI] [PubMed] [Google Scholar]
  16. HURWITZ L., TINSLEY B., BATTLE F. Dissociation of contraction and potassium efflux in smooth muscle. Am J Physiol. 1960 Jul;199:107–111. doi: 10.1152/ajplegacy.1960.199.1.107. [DOI] [PubMed] [Google Scholar]
  17. Hollands B. C., Vanov S. Localization of catechol amines in visceral organs and ganglia of the rat, guinea-pig and rabbit. Br J Pharmacol Chemother. 1965 Oct;25(2):307–316. doi: 10.1111/j.1476-5381.1965.tb02051.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Iversen L. L. The inhibition of noradrenaline uptake by drugs. Adv Drug Res. 1965;2:1–46. [PubMed] [Google Scholar]
  19. JENKINSON D. H., MORTON I. K. EFFECTS OF NORADRENALINE AND ISOPRENALINE ON THE PERMEABILITY OF DEPOLARIZED INTESTINAL SMOOTH MUSCLE TO INORGANIC IONS. Nature. 1965 Jan 30;205:505–506. doi: 10.1038/205505a0. [DOI] [PubMed] [Google Scholar]
  20. Jacobowitz D. Histochemical studies of the autonomic innervation of the gut. J Pharmacol Exp Ther. 1965 Sep;149(3):358–364. [PubMed] [Google Scholar]
  21. Jenkinson D. H., Morton I. K. The effect of noradrenaline on the permeability of depolarized intestinal smooth muscle to inorganic ions. J Physiol. 1967 Feb;188(3):373–386. doi: 10.1113/jphysiol.1967.sp008144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McDOUGAL M. D., WEST G. B. The inhibition of the peristaltic reflex by sympathomimetic amines. Br J Pharmacol Chemother. 1954 Jun;9(2):131–137. doi: 10.1111/j.1476-5381.1954.tb00831.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. NORBERG K. A. ADRENERGIC INNERVATION OF THE INTESTINAL WALL STUDIED BY FLUORESCENCE MICROSCOPY. Int J Neuropharmacol. 1964 Sep;3:379–382. doi: 10.1016/0028-3908(64)90067-x. [DOI] [PubMed] [Google Scholar]
  24. Norberg K. A., Sjöqvist F. New possibilities for adrenergic modulation of ganglionic transmission. Pharmacol Rev. 1966 Mar;18(1):743–751. [PubMed] [Google Scholar]
  25. POWELL C. E., SLATER I. H. Blocking of inhibitory adrenergic receptors by a dichloro analog of isoproterenol. J Pharmacol Exp Ther. 1958 Apr;122(4):480–488. [PubMed] [Google Scholar]
  26. ROBERTSON P. A. Calcium and contractility in depolarized smooth muscle. Nature. 1960 Apr 23;186:316–317. doi: 10.1038/186316a0. [DOI] [PubMed] [Google Scholar]
  27. Schild H. O. Calcium and the relaxant effect of isoproterenol in the depolarized rat uterus. Pharmacol Rev. 1966 Mar;18(1):495–501. [PubMed] [Google Scholar]
  28. WAUGH W. H. Role of calcium in contractile excitation of vascular smooth muscle by epinephrine and potassium. Circ Res. 1962 Dec;11:927–940. doi: 10.1161/01.res.11.6.927. [DOI] [PubMed] [Google Scholar]
  29. van Rossum J. M., Mujić M. Classification of sympathomimetic drugs on the rabbit intestine. Arch Int Pharmacodyn Ther. 1965 Jun;155(2):418–431. [PubMed] [Google Scholar]

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