Skip to main content
NCBI home page
Annals of Surgery logoLink to Annals of Surgery
. 1978 Mar;187(3):303–308. doi: 10.1097/00000658-197803000-00016

The effect of fat on secretin release.

T A Miller, S J Konturek, O L Llanos, P L Rayford, J C Thompson
PMCID: PMC1396421  PMID: 637588

Abstract

The effect of graded doses of intravenously infused secretin and intestinally perfused sodium oleate and HCl on pancreatic exocrine secretion and plasma secretin was determined in cats and dogs prepared with pancreatic fistulas. The pancreatic dose--response curves for bicarbonate following duodenal perfusion of oleate and HCl in cats were almost identical and paralleled the response to exogenous secretin. Although the bicarbonate response to oleate in dogs was less pronounced than the response to HCl or secretin, the bicarbonate output was observed to increase relative to protein output with increasing doses of the intestinally perfused fat. These observations suggested that secretin or a substance with secretin-like activity may be released from the intestine on contact with fat. The inability to detect changes in secretin immunoreactivity in both cats and dogs with increasing doses of oleate suggests that if secretin is released, it is in amounts undetectable by our radioimmunoassay or that some other unknown substance with secretin-like activity may be released.

Full text

PDF
303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbezat G. O., Grossman M. I. Release of cholecystokinin by acid (38562). Proc Soc Exp Biol Med. 1975 Feb;148(2):463–467. doi: 10.3181/00379727-148-38562. [DOI] [PubMed] [Google Scholar]
  2. Bodanszky M., Klausner Y. S., Said S. I. Biological activities of synthetic peptides corresponding to fragments of and to the entire sequence of the vasoactive intestinal peptide. Proc Natl Acad Sci U S A. 1973 Feb;70(2):382–384. doi: 10.1073/pnas.70.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boden G., Essa N., Owen O. E. Effects of intraduodenal amino acids, fatty acids, and sugars on secretin concentrations. Gastroenterology. 1975 Apr;68(4 Pt 1):722–727. [PubMed] [Google Scholar]
  4. Debas H. T., Grossman M. I. Pure cholecystokinin: pancreatic protein and bicarbonate response. Digestion. 1973;9(6):469–481. doi: 10.1159/000197476. [DOI] [PubMed] [Google Scholar]
  5. Herrera F., Kemp D. R., Tsukamoto M., Woodward E. R., Dragstedt L. R. A new cannula for the study of pancreatic function. J Appl Physiol. 1968 Aug;25(2):207–209. doi: 10.1152/jappl.1968.25.2.207. [DOI] [PubMed] [Google Scholar]
  6. Hofmann A. F., Small D. M. Detergent properties of bile salts: correlation with physiological function. Annu Rev Med. 1967;18:333–376. doi: 10.1146/annurev.me.18.020167.002001. [DOI] [PubMed] [Google Scholar]
  7. Konturek S. J., Pucher A., Radecki T. Comparison of vasoactive intestinal peptide and secretin in stimulation of pancreatic secretion. J Physiol. 1976 Feb;255(2):497–509. doi: 10.1113/jphysiol.1976.sp011292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Konturek S. J., Radecki T., Mikos E., Thor J. The effect of exogenous and endogenous secretin and cholecystokinin on pancreatic secretion in cats. Scand J Gastroenterol. 1971;6(5):423–428. doi: 10.3109/00365527109180720. [DOI] [PubMed] [Google Scholar]
  9. Konturek S. J., Thor P., Dembinski A., Krol R. Comparison of secretin and vasoactive intestinal peptide on pancreatic secretion in dogs. Gastroenterology. 1975 Jun;68(6):1527–1535. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Llanos O. L., Swierczek J. S., Miller T. A., Said S. I., Rayford P. L., Thompson J. C. Effect of fat on meal-stimulated gastric and pancreatic secretion. World J Surg. 1977 Jul;1(4):507–512. doi: 10.1007/BF01565926. [DOI] [PubMed] [Google Scholar]
  12. Meyer J. H., Jones R. S. Canine pancreatic responses to intestinally perfused fat and products of fat digestion. Am J Physiol. 1974 May;226(5):1178–1187. doi: 10.1152/ajplegacy.1974.226.5.1178. [DOI] [PubMed] [Google Scholar]
  13. Rayford P. L., Curtis P. J., Fender R., Thompson J. C. Plasma levels of secretin in man and dogs: validation of a secretin radioimmunoassay. Surgery. 1976 Jun;79(6):658–665. [PubMed] [Google Scholar]
  14. Reeder D. D., Becker H. D., Smith N. J., Rayford P. L., Thompson J. C. Measurement of endogenous release of cholecystokinin by radioimmunoassay. Ann Surg. 1973 Sep;178(3):304–310. doi: 10.1097/00000658-197309000-00010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Said S. I., Mutt V. Isolation from porcine-intestinal wall of a vasoactive octacosapeptide related to secretin and to glucagon. Eur J Biochem. 1972 Jul 13;28(2):199–204. doi: 10.1111/j.1432-1033.1972.tb01903.x. [DOI] [PubMed] [Google Scholar]
  16. WANG C. C., GROSSMAN M. I. Physiological determination of release of secretin and pancreozymin from intestine of dogs with transplanted pancreas. Am J Physiol. 1951 Feb;164(2):527–545. doi: 10.1152/ajplegacy.1951.164.2.527. [DOI] [PubMed] [Google Scholar]

Articles from Annals of Surgery are provided here courtesy of Lippincott, Williams, and Wilkins

RESOURCES