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. 2002 Nov;40(11):4405. doi: 10.1128/JCM.40.11.4405.2002

Detection of Smallpox Virus DNA by LightCycler PCR

Mark J Espy 1, Franklin R Cockerill III 1, Richard F Meyer 1, Michael D Bowen 1, Gregory A Poland 1, Ted L Hadfield 1, Thomas F Smith 1
PMCID: PMC139727

Volume 40, no. 6, p. 1985-1988, 2002. Page 1985: The nucleic acid sequences of the primer that was used to amplify the smallpox virus hemagglutinin gene target (GenBank accession no. M14783) and of the probe that was used in the assay are as follows: for the primer, 5′-CTA ATA TCA TTA GTA TAC GCT ACA C-3′ (sense) and 5′-GAG TCG TAA GAT ATT TTA TCC-3′ (antisense), and for the probe, 5′-AAT GAT TAT GTT GTT ATG AGT GCT TG-fluorescein-3′ and 5′-RED 640-TAT AAG GAG CCC AAT TCC ATT ATT CT-PHOS-3′.


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