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. 1972 Mar;22(3):371–380.

A sensitive isotope modification of the mixed haemadsorption test applicable to the study of prozone effects

K G Sundqvist, Astrid Fagraeus
PMCID: PMC1407802  PMID: 4623759

Abstract

Investigations of surface antigens of animal cells require sensitive and quantitative in vitro methods. A modification of the mixed haemadsorption technique (MHT) suitable for such purposes is presented. The indicator cells have been labelled with chromium-51. During studies of experimental conditions, including reproducibility and sensitivity, the technique was used in four model systems including histocompatibility (H-2, HL-A), species, organ and blood group antigens. Isotope labelling of the indicator cells has rendered the test more sensitive and in addition some qualitative properties of antigen—antibody interaction have been studied. The test procedure is simple and fast.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHALMERS D. G., COOMBS R. R., GURNER B. W., DAUSSET J. The mixed antiglobulin reaction in the detection of human isoantibodies against leucocytes, platelets and HeLa cells. Br J Haematol. 1959 Jul;5:225–231. doi: 10.1111/j.1365-2141.1959.tb04030.x. [DOI] [PubMed] [Google Scholar]
  2. COOMBS R. R., BEDFORD D., ROUILLARD L. M. A and B blood-group antigens on human epidermal cells demonstrated by mized agglutination. Lancet. 1956 Apr 21;270(6921):461–463. doi: 10.1016/s0140-6736(56)90528-1. [DOI] [PubMed] [Google Scholar]
  3. Cerottini J. C., Brunner K. T. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence. Immunology. 1967 Oct;13(4):395–403. [PMC free article] [PubMed] [Google Scholar]
  4. ESPMARK J. A., FAGRAEUS A. IDENTIFICATION OF THE SPECIES OF ORIGIN OF CELLS BY MIXED HEMADSORPTION : A MIXED ANTIGLOBULIN REACTION APPLIED TO MONOLAYER CELL CULTURES. J Immunol. 1965 Apr;94:530–537. [PubMed] [Google Scholar]
  5. FAGRAEUS A., ESPMARK A. Use of a 'mixed haemadsorption' method in virus-infected tissue cultures. Nature. 1961 Apr 22;190:370–371. doi: 10.1038/190370a0. [DOI] [PubMed] [Google Scholar]
  6. Fagraeus A., Espmark J. A., Jonsson J. Mixed haemadsorption: a mixed antiglobulin reaction applied to antigens on a glass surface. Preparation and evaluation of indicator red cells; survey of present applications. Immunology. 1965 Aug;9(2):161–175. [PMC free article] [PubMed] [Google Scholar]
  7. Fagraeus A., Jonsson J. Distribution of organ antigens over the surface of thyroid cells as examined by the immunofluorescence test. Immunology. 1970 Mar;18(3):413–416. [PMC free article] [PubMed] [Google Scholar]
  8. Harder F. H., McKhann C. F. Demonstration of cellular antigens on sarcoma cells by an indirect 125-I-labeled antibody technique. J Natl Cancer Inst. 1968 Feb;40(2):231–241. [PubMed] [Google Scholar]
  9. Jonsson J., Fagreaus A. On the mechaism of the ring zone effect obtained with the mixed haemadsorption technique. Studies with human anti-thyroid sera reacting with thyroid monolayer cultures. Immunology. 1969 Sep;17(3):387–411. [PMC free article] [PubMed] [Google Scholar]
  10. Kourilsky F. M., Silvestre D., Levy J. P., Dausset J., Nicolai M. G., Senik A. Immunoferritin study of the distribution of HL-A antigens on human blood cells. J Immunol. 1971 Feb;106(2):454–466. [PubMed] [Google Scholar]
  11. SANDERSON A. R. CYTOTOXIC REACTIONS OF MOUSE ISO-ANTISERA: PRELIMINARY CONSIDERATIONS. Br J Exp Pathol. 1964 Aug;45:398–408. [PMC free article] [PubMed] [Google Scholar]
  12. Sparks F. C., Ting C. C., Hammond W. G., Herberman R. B. An isotopic antiglobulin technique for measuring antibodies to cell-surface antigens. J Immunol. 1969 Apr;102(4):842–847. [PubMed] [Google Scholar]
  13. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]

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