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. 1972 Mar;22(3):485–491.

Xenogeneic recognition of tumour specific plasma membrane antigens derived from mouse lymphoma cells

Anneliese Wolf, Rita K Barfoot, R A Johnson
PMCID: PMC1407810  PMID: 5026856

Abstract

Rabbit antisera were prepared to two mouse ascitic lymphomas, one of them being highly immunogenic, the other one weakly immunogenic in syngeneic hosts. A comparison was made between antisera raised with intact cells and antisera raised with cell-free fractions composed of purified large plasma membranes. The antisera were tested in vitro for their lytic capacity by the 51Cr-release technique, and in vivo for their capacity to protect syngeneic mice against a tumour challenge. The antisera were used unabsorbed, absorbed with syngeneic mouse tissue, and also after passage through syngeneic animals. The antisera with the highest specificity for the respective tumour in mice were those raised by plasma membranes derived from the strongly immunogenic tumour. Lysis in the 51Cr test corresponded roughly with these findings. Xenogeneic recognition of tumour distinct antigens as well as the therapeutic value of xenogeneic antisera against established lymphomas growing in the ascitic form are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOS D. B. The persistence of mouse iso-antibodies in vivo. Br J Cancer. 1955 Mar;9(1):216–221. doi: 10.1038/bjc.1955.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alexander P., Connell D. I., Mikulska Z. B. Treatment of a murine leukemia with spleen cells or sera from allogeneic mice immunized against the tumor. Cancer Res. 1966 Jul;26(7):1508–1515. [PubMed] [Google Scholar]
  3. GORER P. A., AMOS D. B. Passive immunity in mice against C57BL leukosis E.L. 4 by means of iso-immune serum. Cancer Res. 1956 May;16(4):338–343. [PubMed] [Google Scholar]
  4. HERBERMAN R., STETSON C. A., Jr THE EXPRESSION OF HISTOCOMPATIBILITY ANTIGENS ON CELLULAR AND SUBCELLULAR MEMBRANES. J Exp Med. 1965 Apr 1;121:533–549. doi: 10.1084/jem.121.4.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Levi E., Golden R., Zerubavel R., Fisher J. The activity of tolerant antileukemia sera. I. In vivo effects. Cancer Res. 1966 Apr;26(4):659–667. [PubMed] [Google Scholar]
  6. MOHOS S. C., KIDD J. G. Effects of various immune rabbit serums on the cells of several transplanted mouse lymphomas in vitro and in vivo; with special reference to inhibition by an antibody that reacts with neoplastic and non-neoplastic lymphocytic cells of mice. J Exp Med. 1957 Mar 1;105(3):233–263. doi: 10.1084/jem.105.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller D. G., Moldovanu G., Kaplan A., Tocci S. Antilymphocytic leukemic serum and chemotherapy in the treatment of murine leukemia. Cancer. 1968 Dec;22(6):1192–1198. doi: 10.1002/1097-0142(196811)22:6<1192::aid-cncr2820220615>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  8. Reif A. E., Kim C. A. Therapy of transplantable mouse leukaemias with antileukaemia sera. Nature. 1969 Sep 27;223(5213):1377–1379. doi: 10.1038/2231377a0. [DOI] [PubMed] [Google Scholar]
  9. Shigeno N., Hämmerling U., Arpels C., Boyse E. A., Old L. J. Preparation of lymphocyte-specific antibody from anti-lymphocyte serum. Lancet. 1968 Aug 10;2(7563):320–323. doi: 10.1016/s0140-6736(68)90530-8. [DOI] [PubMed] [Google Scholar]
  10. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
  11. Wolf A., Avis P. J. Preparation and purification of plasma membranes from murine lymphoma cells carrying tumour-specific antigenicity. Transplantation. 1970 Jan;9(1):18–29. doi: 10.1097/00007890-197001000-00003. [DOI] [PubMed] [Google Scholar]

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