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. 1972 Jun;22(6):991–999.

Biosynthesis of the second (C2) and fourth (C4) components of complement in vitro by tissues isolated from guinea-pigs with genetically determined C4 deficiency*

H R Colten, M M Frank
PMCID: PMC1407873  PMID: 5032493

Abstract

Tissues and cells isolated from guinea-pigs homozygous for a genetically determined deficiency of the fourth (C4) component of complement failed to synthesize C4 and synthesized C2 at a rate approximately 40 per cent of normal. These abnormalities in the biosynthesis of C2 and C4 persisted up to four weeks in tissue culture. Tissues and cells from heterozygous C4-deficient animals produced C4 at a rate intermediate between normal and that of the homozygous deficient. These observations demonstrate that the reduced serum concentrations of C2 and C4 in C4-deficient guinea-pigs can be accounted for by abnormalities in biosynthesis and are probably not the result of hypercatabolism of these proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper C. A., Propp R. P., Klemperer M. R., Rosen F. S. Inherited deficiency of the third component of human complement (C'3). J Clin Invest. 1969 Mar;48(3):553–557. doi: 10.1172/JCI106013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DONALDSON V. H., EVANS R. R. A BIOCHEMICAL ABNORMALITY IN HEREDIATRY ANGIONEUROTIC EDEMA: ABSENCE OF SERUM INHIBITOR OF C' 1-ESTERASE. Am J Med. 1963 Jul;35:37–44. doi: 10.1016/0002-9343(63)90162-1. [DOI] [PubMed] [Google Scholar]
  3. Ellman L., Green I., Frank M. Genetically controlled total deficiency of the fourth component of complement in the guinea pig. Science. 1970 Oct 2;170(3953):74–75. doi: 10.1126/science.170.3953.74. [DOI] [PubMed] [Google Scholar]
  4. Frank M. M., May J., Gaither T., Ellman L. In vitro studies of complement function in sera of C4-deficient guinea pigs. J Exp Med. 1971 Jul 1;134(1):176–187. doi: 10.1084/jem.134.1.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klemperer M. R., Woodworth H. C., Rosen F. S., Austen K. F. Hereditary deficiency of the second component of complement (C'2) in man. J Clin Invest. 1966 Jun;45(6):880–890. doi: 10.1172/JCI105403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Opferkuch W., Rapp H. J., Colten H. R., Borsos T. Immune hemolysis and the functional properties of the second (C2) and fourth (C4) components of complement. 3. The hemolytic efficiency of human and guinea pig C2 and C4. J Immunol. 1971 Apr;106(4):927–931. [PubMed] [Google Scholar]
  7. ROSENBERG L. T., TACHIBANA D. K. Activity of mouse complement. J Immunol. 1962 Dec;89:861–867. [PubMed] [Google Scholar]
  8. Rother K., Rother U., Müller-Eberhard H. J., Nilsson J. R. Deficiency of the sixth component of complement in rabbits with an inherited complement defect. J Exp Med. 1966 Oct 1;124(4):773–785. doi: 10.1084/jem.124.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rubin D. J., Borsos T., Rapp H. J., Colten H. R. Synthesis of the second component of guinea pig complement in vitro. J Immunol. 1971 Feb;106(2):295–303. [PubMed] [Google Scholar]

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