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. 1972 Jun;22(6):967–980.

Antigen-binding thymus-derived lymphocytes

II. Nature of the immunoglobulin determinants

Nancy M Hogg, M F Greaves
PMCID: PMC1407883  PMID: 4113387

Abstract

Thymus-derived `rosette'-forming lymphocytes which have been separated from other SRBC-sensitive cells by means of cotton wool columns were examined for the presence of immunoglobulin. This was carried out by inhibition of rosette formation by anti-immunoglobulin sera. Inhibition was effected by a number of anti-IgM sera shown to contain antibodies with specificities directed towards the `hinge' region of the μ chain. No other heavy chain specific antisera were inhibitory.

The ratio of rosette inhibition by anti-κ and anti-λ light chain sera varied during the course of the response to SRBC, the latter inhibiting by 89 per cent 3 days post-immunization.

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Selected References

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