Abstract
Anaphylactic hypersensitivity of isolated guinea-pig ileum preparations has been evaluated by investigation of antigen-dose/response relationships, in vitro. The influence of Freund's adjuvants on active sensitization has been investigated. In contrast to the inhibitory influence previously observed in vivo, the degree of hypersensitivity of isolated tissues in vitro was potentiated by the adjuvants. It is thought that high circulating titres of antibody in vivo exerted a protective or neutralizing effect in relation to challenging doses of antigen. Isolated tissues were freed from this influence. Tissues sensitized with the aid of Freund's complete adjuvant (FCA) revealed the influence of other inhibitory mechanisms, due probably to interaction of various antibody types.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Binaghi R. A. Production of 7 S immunoglobulins in immunized guinea pigs. J Immunol. 1966 Aug;97(2):159–164. [PubMed] [Google Scholar]
- Colquhoun D., Brocklehurst W. E. Passive sensitization of skin and lung by guinea-pig immunoglobulins. Immunology. 1965 Dec;9(6):591–611. [PMC free article] [PubMed] [Google Scholar]
- Dale M. M. The applicability of anaphylactic tests in studies of antigen mixtures. II. The discriminatory capacity of the tests. Immunology. 1965 May;8(5):444–453. [PMC free article] [PubMed] [Google Scholar]
- HALPERN B. N., LIACOPOULOS P., LIACOPOULOS-BRIOT M., BINAGHI R., VAN NEER F. Patterns of in vitro sensitization of isolated smooth muscle tissues with precipitating antibody. Immunology. 1959 Oct;2:351–362. [PMC free article] [PubMed] [Google Scholar]
- Hicks R., Skeldon N. The influence of adjuvants on antibody production and anaphylactic hypersensitivity in the guinea pig. Int Arch Allergy Appl Immunol. 1970;39(2-3):234–246. doi: 10.1159/000230351. [DOI] [PubMed] [Google Scholar]
- Okpako D. T. Antigen dose-response relationships in guinea-pig ileum anaphylaxis. Int Arch Allergy Appl Immunol. 1970;38(5):474–481. doi: 10.1159/000230300. [DOI] [PubMed] [Google Scholar]
- WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]