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. 1972 Aug;23(2):215–224.

Ultrastructural changes in guinea-pig bone marrow basophils during anaphylaxis

Beatrice S T Chan
PMCID: PMC1407908  PMID: 4115968

Abstract

A sequence of ultrastructural changes related to the degrees of severity of the anaphylactic reaction was observed in the bone marrow basophils of guinea-pigs. This sequence of changes might represent the sequential stages of a process of degranulation which consisted of (1) formation of haloes of low electron-density around the granules, (2) development of vacuoles by intercommunicating the adjacent haloes and of confluent vacuoles by joining the small vacuoles, and (3) disruption of cell membrane with concomitant expulsion of granules into the intercellular space or sinusoid. The vacuoles might communicate with the cisternae of the endoplasmic reticulum. Degranulation was found to be a result of an antigen—antibody reaction in which the reaginic antibodies produced had a selective affinity for the cell membrane of the basophils. The mechanism of degranulation might involve two factors: a physicochemical factor of changing the nature of the cell membrane resulting from the antigen—antibody interaction, and a mechanical factor of pressure exerted on the cell membrane by the confluent vacuoles. The alterations in the granules themselves, such as relatively larger size, lighter electron-opacity and looser texture, appeared to be associated with the histamine release from the granules. The structural changes of the granule contents encountered in anaphylaxis suggest that the ultrastructure of the basophil granules may be composed of an orderly interlacing framework of microfilaments onto which other constituent substances are bound.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERMAN G. A. Cytochemical properties of the blood basophilic granulocyte. Ann N Y Acad Sci. 1963 Feb 26;103:376–393. doi: 10.1111/j.1749-6632.1963.tb53710.x. [DOI] [PubMed] [Google Scholar]
  2. ALBANUS L., WINQVIST G. Basophils, eosinophils and histamine in the bone marrow of the guinea pig. Acta Haematol. 1961;26:365–371. doi: 10.1159/000206676. [DOI] [PubMed] [Google Scholar]
  3. Bergquist G., Nilzén A. The release of histamine from blood corpuscles by antigen-antibody reactions. I. A method for detecting allergy in vitro in atopic persons. Acta Allergol. 1968 Oct;23(5):363–373. doi: 10.1111/j.1398-9995.1968.tb04069.x. [DOI] [PubMed] [Google Scholar]
  4. Bergquist G., Nilzén A. The release of histamine from blood corpuscles by antigen-antibody reactions. II. The histamine release from whole blood after adding specific allergen in hyposensitized persons. Acta Allergol. 1969 Nov;24(4):261–267. doi: 10.1111/j.1398-9995.1969.tb03741.x. [DOI] [PubMed] [Google Scholar]
  5. Bloom G. D., Fredholm B., Haegermark O. Studies on the time course of histamine release and morphological changes induced by histamine liberators in rat peritoneal mast cells. Acta Physiol Scand. 1967 Dec;71(4):270–282. doi: 10.1111/j.1748-1716.1967.tb03734.x. [DOI] [PubMed] [Google Scholar]
  6. Bloom G. D., Haegermark O. A study on morphological changes and histamine release induced by compound 48/80 in rat peritoneal mast cells. Exp Cell Res. 1965 Dec;40(3):637–654. doi: 10.1016/0014-4827(65)90241-7. [DOI] [PubMed] [Google Scholar]
  7. Bloom G. D., Haegermark O. Studies on morphological changes and histamine release induced by bee venom, n-decylamine and hypotonic solutions in rat peritoneal mast cells. Acta Physiol Scand. 1967 Dec;71(4):257–269. doi: 10.1111/j.1748-1716.1967.tb03733.x. [DOI] [PubMed] [Google Scholar]
  8. CARTER P. B., HIGGINBOTHAM R. D., DOUGHERTY T. F. The local response of tissue mast cells to antigen in sensitized mice. J Immunol. 1957 Sep;79(3):259–264. [PubMed] [Google Scholar]
  9. CASS R., MARSHALL P. B., RILEY J. F. 5-Hydroxytryptamine and histamine in mast cells of the mouse and rat. J Physiol. 1958 May 28;141(3):510–519. doi: 10.1113/jphysiol.1958.sp005992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. CODE C. F., MITCHELL R. G. Histamine, eosinophils and basophils in the blood. J Physiol. 1957 May 23;136(3):449–468. doi: 10.1113/jphysiol.1957.sp005773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chakravarty N., Gustafson G. T., Pihl E. Ultrastructural changes in rat mast cells during anaphylactic histamine release. Acta Pathol Microbiol Scand. 1967;71(2):233–244. doi: 10.1111/j.1699-0463.1967.tb05161.x. [DOI] [PubMed] [Google Scholar]
  12. Chan B. S. Electron microscopic study of the granules in guinea-pig bone marrow basophils. Acta Haematol. 1969;42(5):258–268. doi: 10.1159/000208809. [DOI] [PubMed] [Google Scholar]
  13. Combs J. W. Maturation of rat mast cells. An electron microscope study. J Cell Biol. 1966 Dec;31(3):563–575. doi: 10.1083/jcb.31.3.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. FAWCETT D. W. Cytological and pharmacological observations on the release of histamine by mast cells. J Exp Med. 1954 Aug 1;100(2):217–224. doi: 10.1084/jem.100.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GRAHAM H. T., LOWRY O. H., WAHL N., PRIEBAT M. K. Mast cells as sources of tissue histamine. J Exp Med. 1955 Sep 1;102(3):307–318. doi: 10.1084/jem.102.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. GRAHAM H. T., LOWRY O. H., WHEELWRIGHT F., LENZ M. A., PARISH H. H., Jr Distribution of histamine among leukocytes and platelets. Blood. 1955 May;10(5):467–481. [PubMed] [Google Scholar]
  17. HUMPHREY J. H., MOTA I. The mechanism of anaphylaxis: specificity of antigen-induced mast cell damage in anaphylaxis in the guinea pig. Immunology. 1959 Jan;2(1):31–43. [PMC free article] [PubMed] [Google Scholar]
  18. Hastie R. The antigen-induced degranulation of basophil leucocytes from atopic subjects, studied by phase-contrast microscopy. Clin Exp Immunol. 1971 Jan;8(1):45–61. [PMC free article] [PubMed] [Google Scholar]
  19. Krüger P. G., Diamant B., Scholander L. Non-degranulating structural changes of rat mast cells induced by antigen and toluidine blue. Exp Cell Res. 1970 Nov;63(1):101–109. doi: 10.1016/0014-4827(70)90336-8. [DOI] [PubMed] [Google Scholar]
  20. LAGUNOFF D., PHILLIPS M., BENDITT E. P. The histochemical demonstration of histamine in mast cells. J Histochem Cytochem. 1961 Sep;9:534–541. doi: 10.1177/9.5.534. [DOI] [PubMed] [Google Scholar]
  21. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Logan G. B. Blood and tissue histamine during in vivo anaphylactic shock in susceptible and nonsusceptible guinea pigs. Int Arch Allergy Appl Immunol. 1971;40(3):419–427. doi: 10.1159/000230424. [DOI] [PubMed] [Google Scholar]
  23. MOTA I. Action of anaphylactic shock and anaphylatoxin on mast cells and histamine in rats. Br J Pharmacol Chemother. 1957 Dec;12(4):453–456. doi: 10.1111/j.1476-5381.1957.tb00164.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. MOTA I. Effect of antigen and octylamine on mast cells and histamine content of sensitized guinea-pig tissues. J Physiol. 1959 Oct;147:425–436. doi: 10.1113/jphysiol.1959.sp006253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. MOTA I. Mast cell and histamine in rat anaphylaxis: the effect of Haemophilus pertussis. Nature. 1958 Oct 11;182(4641):1021–1022. doi: 10.1038/1821021a0. [DOI] [PubMed] [Google Scholar]
  26. MOTA I. THE BEHAVIOR OF MAST CELLS IN ANAPHYLAXIS. Int Rev Cytol. 1963;15:363–397. doi: 10.1016/s0074-7696(08)61121-3. [DOI] [PubMed] [Google Scholar]
  27. MOTA I., VUGMAN I. Effects of anaphylactic shock and compound 48/80 on the mast cells of the guinea pig lung. Nature. 1956 Mar 3;177(4505):427–429. doi: 10.1038/177427a0. [DOI] [PubMed] [Google Scholar]
  28. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. RILEY J. F. Heparin, histamine and mast cells. Blood. 1954 Dec;9(12):1123–1126. [PubMed] [Google Scholar]
  30. RILEY J. F. The effects of histamine-liberators on the mast cells of the rat. J Pathol Bacteriol. 1953 Apr;65(2):471–479. doi: 10.1002/path.1700650219. [DOI] [PubMed] [Google Scholar]
  31. RILEY J. F., WEST G. B. The presence of histamine in tissue mast cells. J Physiol. 1953 Jun 29;120(4):528–537. doi: 10.1113/jphysiol.1953.sp004915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. SHELLEY W. B., JUHLIN L. Functional cytology of the human basophil in allergic and physiologic reactions: technic and atlas. Blood. 1962 Feb;19:208–216. [PubMed] [Google Scholar]
  33. Sampson D., Archer G. T. Release of histamine from human basophils. Blood. 1967 May;29(5):722–736. [PubMed] [Google Scholar]
  34. Singleton E. M., Clark S. L., Jr The response of mast cells to compound 48/80 studied with the electron microscope. Lab Invest. 1965 Oct;14(10):1744–1763. [PubMed] [Google Scholar]
  35. Taichman N. S. Ultrastructural alterations in guinea pig mast cells during anaphylaxis. Int Arch Allergy Appl Immunol. 1971;40(6):934–942. doi: 10.1159/000230475. [DOI] [PubMed] [Google Scholar]
  36. Terry R. W., Bainton D. F., Farquhar M. G. Formation and structure of specific granules in basophilic leukocytes of the guinea pig. Lab Invest. 1969 Jul;21(1):65–76. [PubMed] [Google Scholar]
  37. VALENTINE W. N., LAWRENCE J. S., PEARCE M. L., BECK W. S. The relationship of the basophil to blood histamine in man. Blood. 1955 Feb;10(2):154–159. [PubMed] [Google Scholar]
  38. WAALKES T. P., WEISSBACH H., BOZICEVICH J., UDENFRIEND S. Serotonin and histamine release during anaphylaxis in the rabbit. J Clin Invest. 1957 Jul;36(7):1115–1120. doi: 10.1172/JCI103507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. WINQVIST G. Electron microscopy of the basophilic granulocyte. Ann N Y Acad Sci. 1963 Feb 26;103:352–375. doi: 10.1111/j.1749-6632.1963.tb53709.x. [DOI] [PubMed] [Google Scholar]
  40. Wetzel B. K., Horn R. G., Spicer S. S. Fine structural studies on the development of heterophil, eosinophil, and basophil granulocytes in rabbits. Lab Invest. 1967 Mar;16(3):349–382. [PubMed] [Google Scholar]

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