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. 1972 Sep;23(3):449–463.

Polymorphism and intraspecific differences among spermatozoan and seminal plasma antigens

A C Kulangara
PMCID: PMC1407944  PMID: 4139114

Abstract

Ram SS (supernatant fraction of spermatozoa ground with Baker's fluid) and SP (seminal plasma) were analysed by double diffusion and immunoelectrophoresis in agar using six different rabbit antisera.

Immunoelectrophoretic patterns of SS from ten rams show frequencies of band subpatterns which indicate polymorphism of spermatozoan antigens. Also, bands from different rams with the same diffusion characteristics but different electrophoretic mobility and others with different antigenic determinants confirm protein polymorphism.

SS and SP of three rams from Blackface, Dorset and Merino breeds were examined by agar diffusion and immunoelectrophoresis at pH 5 and 8.2. Five to six spermatozoan antigens were distinguished from 4–5 SP antigens. When similar antigens from two rams were further tested, it was possible to show some difference in electrophoretic mobility or antigenic determinant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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