Abstract
Denatured calf and rat skin collagen and α1- and α2-chains from these collagens were tested for their capacity to sensitize guinea-pigs for the cutaneous delayed hypersensitivity reaction and to elicit this reaction in sensitized animals. Alpha-chains from neutral salt extracted and from urea extracted collagen were also compared. All preparations were fully active as sensitizers as well as elicitors of the skin reaction. In spite of extensive cross-reactions, significant differences between α1- and α2-chains of a given species as well as between corresponding chains of different species could be detected. Differences between chains from neutral salt extracted collagen and from urea extracted collagen were also revealed.
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Selected References
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- Davison P. F., Levine L., Drake M. P., Rubin A., Bump S. The serologic specificity of tropocollagen telopeptides. J Exp Med. 1967 Aug 1;126(2):331–346. doi: 10.1084/jem.126.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lindsley H., Mannik M., Bornstein P. The distribution of antigenic determinants in rat skin collagen. J Exp Med. 1971 Jun 1;133(6):1309–1324. doi: 10.1084/jem.133.6.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meigel W., Pontz B., Timpl R., Hieber E., Nordwig A., Steffen C., Kühn K. Comparative immunologic studies on fish and invertebrate collagens. J Immunol. 1971 Oct;107(4):1146–1151. [PubMed] [Google Scholar]
- Michaeli D., Martin G. R., Kettman J., Benjamini E., Leung D. Y., Blatt B. A. Localization of antigenic determinants in the polypeptide chains of collagen. Science. 1969 Dec 19;166(3912):1522–1524. doi: 10.1126/science.166.3912.1522. [DOI] [PubMed] [Google Scholar]
- Pontz B., Meigel W., Rauterberg J., Kühn K. Localization of two species specific antigenic determinants on the peptide chains of calf skin collagen. Eur J Biochem. 1970 Sep;16(1):50–54. doi: 10.1111/j.1432-1033.1970.tb01051.x. [DOI] [PubMed] [Google Scholar]
- Timpl R., Beil W., Furthmayr H., Meigel W., Pontz B. Characterization of conformation independent antigenic determinants in the triple-helical part of calf and rat collagen. Immunology. 1971 Dec;21(6):1017–1030. [PMC free article] [PubMed] [Google Scholar]