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. 1972 Nov;23(5):705–711.

Complement and phospholipase C lysis of lipid membranes

T R Hesketh, Sheila N Payne, J H Humphrey
PMCID: PMC1407964  PMID: 4673869

Abstract

Disruption by activated complement of liposomal membranes containing phospholipids can occur whether or not cholesterol is present and whether or not the phospholipids contain unsaturated fatty acid residues. Certain membrane structures, susceptible to the action of complement, were not disrupted by phospholipase C. We conclude that the terminal stages of complement lysis do not involve enzymatic cleavage of the C-O-P linkage in phospholipids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dalmasso A. P., Müller-Eberhard H. J. Hemolytic activity of lipoprotein-depleted serum and the effect of certain anions on complement. J Immunol. 1966 Nov;97(5):680–685. [PubMed] [Google Scholar]
  2. Hakomori S., Strycharz G. D. Investigations on cellular blood-group substances. I. Isolation and chemical composition of blood-group ABH and Le-b isoantigens of sphingoglycolipid nature. Biochemistry. 1968 Apr;7(4):1279–1286. doi: 10.1021/bi00844a005. [DOI] [PubMed] [Google Scholar]
  3. Hesketh T. R., Dourmashkin R. R., Payne S. N., Humphrey J. H., Lachmann P. J. Lesions due to complement in lipid membranes. Nature. 1971 Oct 29;233(5322):620–623. doi: 10.1038/233620a0. [DOI] [PubMed] [Google Scholar]
  4. Humphrey J. H., Dourmashkin R. R. The lesions in cell membranes caused by complement. Adv Immunol. 1969;11:75–115. doi: 10.1016/s0065-2776(08)60478-2. [DOI] [PubMed] [Google Scholar]
  5. Inoue K., Kinsky S. C. Fate of phospholipids in liposomal model membranes damaged by antibody and complement. Biochemistry. 1970 Nov 24;9(24):4767–4776. doi: 10.1021/bi00826a021. [DOI] [PubMed] [Google Scholar]
  6. Keller R. Anaphylaxis in isolated rat mast cells. IV. Studies on the possible role of lysolecithin as an agent responsible for immune cellular injury. Int Arch Allergy Appl Immunol. 1965;28(4):201–216. [PubMed] [Google Scholar]
  7. Kinsky S. C., Bonsen P. P., Kinsky C. B., Van Deenen L. L., Rosenthal A. F. Preparation of immunologically responsive liposomes with phosphonyl and phosphinyl analogs of lecithin. Biochim Biophys Acta. 1971 Jun 1;233(3):815–819. doi: 10.1016/0005-2736(71)90183-0. [DOI] [PubMed] [Google Scholar]
  8. Kinsky S. C., Haxby J. A., Zopf D. A., Alving C. R., Kinsky C. B. Complement-dependent damage to liposomes prepared from pure lipids and Forssman hapten. Biochemistry. 1969 Oct;8(10):4149–4158. doi: 10.1021/bi00838a036. [DOI] [PubMed] [Google Scholar]
  9. Knudson K. C., Bing D. H., Kater L. Quantitative measurement of guinea pig complement with liposomes. J Immunol. 1971 Jan;106(1):258–265. [PubMed] [Google Scholar]
  10. Lachmann P. J., Munn E. A., Weissmanng Complement-mediated lysis of liposomes produced by the reactive lysis procedure. Immunology. 1970 Dec;19(6):983–986. [PMC free article] [PubMed] [Google Scholar]
  11. Lachmann P. J., Thompson R. A. Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9. J Exp Med. 1970 Apr 1;131(4):643–657. doi: 10.1084/jem.131.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MOORE J. H., WILLIAMS D. L. SOME OBSERVATIONS ON THE SPECIFICITY OF PHOSPHOLIPASE A. Biochim Biophys Acta. 1964 Feb 24;84:41–54. doi: 10.1016/0926-6542(64)90099-x. [DOI] [PubMed] [Google Scholar]
  13. Roelofsen B., Zwaal R. F., Comfurius P., Woodward C. B., van Deenen L. L. Action of pure phospholipase A 2 and phospholipase C on human erythrocytes and ghosts. Biochim Biophys Acta. 1971 Sep 14;241(3):925–929. doi: 10.1016/0005-2736(71)90024-1. [DOI] [PubMed] [Google Scholar]
  14. Thompson R. A., Lachmann P. J. Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7. J Exp Med. 1970 Apr 1;131(4):629–641. doi: 10.1084/jem.131.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van DEENEN L., de HAAS G., HEEMSKERK C. H. Hydrolysis of synthetic mixed-acid phosphatides by phospholipase A from human pancreas. Biochim Biophys Acta. 1963 Feb 12;67:295–304. [PubMed] [Google Scholar]
  16. van DEENEN L., de HAAS G., HEEMSKERK C. H., MEDUSKI J. Hydrolysis of synthetic phosphatides by Clostridium welchii phosphatidase. Biochem Biophys Res Commun. 1961 Mar 10;4:183–188. doi: 10.1016/0006-291x(61)90267-4. [DOI] [PubMed] [Google Scholar]

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