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. 1972 Oct;23(4):615–626.

Role of macrophages in tumour immunity

I. Co-operation between macrophages and lymphoid cells in syngeneic tumour immunity

R Evans, P Alexander
PMCID: PMC1407972  PMID: 5084104

Abstract

Macrophages from DBA/2 mice hyperimmunized with irradiated syngeneic L5178Y or SL2 lymphoma cells inhibited the growth of these cells in vitro and killed them within 48 hours of culture. The reaction was immunologically specific. Non-immune macrophages could be rendered capable of immunologically-specific growth inhibition of target cells in vitro (arming) (a) by direct contact of hyperimmune lymphoid cells and macrophages, and (b) by incubating macrophages with a specific macrophage-arming factor (SMAF) derived by incubating spleen cells from singly immunized mice with irradiated lymphoma cells. Singly immunized spleen cells did not arm macrophages by direct cell-to-cell contact. A temporal relationship was seen between the presence of immune macrophages in hyperimmunized mice and the ability of the spleen cells to arm normal macrophages. Furthermore, macrophages could be armed in vivo by a single i.p. injection of hyper immune spleen cells. The presence of arming factors cytophilic for macrophages, but not for cells of non-macrophage origin, is briefly discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P., Connell D. I., Mikulska Z. B. Treatment of a murine leukemia with spleen cells or sera from allogeneic mice immunized against the tumor. Cancer Res. 1966 Jul;26(7):1508–1515. [PubMed] [Google Scholar]
  2. EHRMANN R. L., GEY G. O. The growth of cells on a transparent gel of reconstituted rat-tail collagen. J Natl Cancer Inst. 1956 Jun;16(6):1375–1403. [PubMed] [Google Scholar]
  3. Evans R., Alexander P. Cooperation of immune lymphoid cells with macrophages in tumour immunity. Nature. 1970 Nov 14;228(5272):620–622. doi: 10.1038/228620a0. [DOI] [PubMed] [Google Scholar]
  4. Evans R., Alexander P. Rendering macrophages specifically cytotoxic by a factor released from immune lymphoid cells. Transplantation. 1971 Sep;12(3):227–229. doi: 10.1097/00007890-197109000-00015. [DOI] [PubMed] [Google Scholar]
  5. Evans R. Cultivation and in vitro characterization of mouse phagocytic cells. J Reticuloendothel Soc. 1970 Dec;8(6):571–581. [PubMed] [Google Scholar]
  6. Evans R. Phagocytosis of murine lymphoma cells by macrophages. II. Differences between opsonic and cytotoxic activity of mice immunized with lymphoma cells. Immunology. 1971 Jan;20(1):75–83. [PMC free article] [PubMed] [Google Scholar]
  7. GORER P. A. Some recent work on tumor immunity. Adv Cancer Res. 1956;4:149–186. doi: 10.1016/s0065-230x(08)60724-1. [DOI] [PubMed] [Google Scholar]
  8. GRANGER G. A., WEISER R. S. HOMOGRAFT TARGET CELLS: SPECIFIC DESTRUCTION IN VITRO BY CONTACT INTERACTION WITH IMMUNE MACROPHAGES. Science. 1964 Sep 25;145(3639):1427–1429. doi: 10.1126/science.145.3639.1427. [DOI] [PubMed] [Google Scholar]
  9. Hoy W. E., Nelson D. S. Studies on cytophilic antibodies. V. Alloantibodies cytophilic for mouse macrophages. Aust J Exp Biol Med Sci. 1969 Oct;47(5):525–539. doi: 10.1038/icb.1969.147. [DOI] [PubMed] [Google Scholar]

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