Abstract
The histogram of electrophoretic mobilities (EPM) of normal rat lymph node cells indicates a heterogeneous population. It can be resolved into two independent components with Gaussian distribution profiles. These two, Class `A' cells and Class `B' cells have different mean EPM. Their response in vivo to an antigenic stimulation also appears to be different. Neither the mean EPM nor the relative concentration of `B' cells shows any change. The mean EPM of `A' cells also remains constant but their relative concentration decreases significantly with a concomitant emergence of a new cell type with a much lower mean EPM. The relative concentration of this new cell type varies with time but its kinetics parallels that of the circulating antibody titre.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
- EVERETT N. B., CAFFREY R. W., RIEKE W. O. RECIRCULATION OF LYMPHOCYTES. Ann N Y Acad Sci. 1964 Feb 28;113:887–897. doi: 10.1111/j.1749-6632.1964.tb40710.x. [DOI] [PubMed] [Google Scholar]
- Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
- Ruhenstroth-Bauer G., Lücke-Huhle C. Two populations of small lymphocytes. J Cell Biol. 1968 Apr;37(1):196–199. doi: 10.1083/jcb.37.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sundaram K., Phondke G. P., Ambrose E. J. Electrophoretic mobilities of antigen-stimulated lymph node cells. Immunology. 1967 Jan;12(1):21–26. [PMC free article] [PubMed] [Google Scholar]
- Sundaram K., Phondke G. P., Sundaresan P. In vitro studies on antibody production by lymph node cells using cell electrophoresis. Immunology. 1967 Oct;13(4):433–439. [PMC free article] [PubMed] [Google Scholar]