Abstract
Some mouse anti-sheep-erythrocyte macrophage cytophilic antibodies are more readily adsorbed to cells at 4° than at 37°. By treating cells with serum at 4°, washing them well and eluting bound material at 30°, it was found possible to isolate cytophilic immunoglobulins. These were found to have fast γ-mobility, but were heterogeneous upon preparative ultracentrifugation and gel-filtration. The macrophage eluate contained relatively high titres of anti-sheep-erythrocyte cytophilic antibodies.
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