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. 1972 Jan;22(1):141–145.

Cell-mediated immunity in periodontal disease; cytotoxicity, migration inhibition and lymphocyte transformation studies

L Ivanyi, J M A Wilton, T Lehner
PMCID: PMC1408213  PMID: 5013909

Abstract

Cultures of peripheral blood lymphocytes from patients with gingivitis or mild periodontitis were stimulated with ultrasonicates of Veillonella alcalescens to yield an increase of 14C-thymidine incorporation and of cytotoxicity against 51Cr-labelled chicken red cells. The cell-free supernatant from the antigen-stimulated cultures inhibited migration of guinea-pig peritoneal macrophages. In patients with severe periodontitis lymphocytes showed only weak transformation but strong cytotoxic and migration-inhibitory activity. These data suggest that cell-mediated immunity against an oral Gram-negative micro-organism plays some part in the pathogenesis of periodontal disease. The advanced stage of disease, however, revealed a dissociation between lymphocyte transformation and cytotoxicity or macrophage inhibition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaparas S. D., Thor D. E., Godfrey H. P., Baer H., Hedrick S. R. Tuberculin-active carbohydrate that induces inhibition of macrophage migration but not lymphocyte transformation. Science. 1970 Nov 6;170(3958):637–639. doi: 10.1126/science.170.3958.637. [DOI] [PubMed] [Google Scholar]
  2. Holm G., Perlmann P. Cytotoxic potential of stimulated human lymphocytes. J Exp Med. 1967 Apr 1;125(4):721–736. doi: 10.1084/jem.125.4.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holm G. The in vitro cytotoxicity of human lymphocytes: the effect of metabolic inhibitors. Exp Cell Res. 1967 Nov;48(2):334–349. doi: 10.1016/0014-4827(67)90359-x. [DOI] [PubMed] [Google Scholar]
  4. Ivanyi L., Lehner T. Lymphocyte transformation by sonicates of dental plaque in human periodontal disease. Arch Oral Biol. 1971 Sep;16(9):1117–1121. doi: 10.1016/0003-9969(71)90216-0. [DOI] [PubMed] [Google Scholar]
  5. Ivanyi L., Lehner T. Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease. Arch Oral Biol. 1970 Nov;15(11):1089–1096. doi: 10.1016/0003-9969(70)90121-4. [DOI] [PubMed] [Google Scholar]
  6. Lundgren G., Möller G. Non-specific induction of cytotoxicity in normal human lymphocytes in vitro: studies of mechanism and specificity of the reaction. Clin Exp Immunol. 1969 Apr;4(4):435–452. [PMC free article] [PubMed] [Google Scholar]
  7. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  8. Perlmann P., Perlmann H., Holm G. Cytotoxic action of stimulated lymphocytes on allogenic and autologous erythrocytes. Science. 1968 Apr 19;160(3825):306–309. doi: 10.1126/science.160.3825.306. [DOI] [PubMed] [Google Scholar]
  9. Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
  10. Thor D. E., Jureziz R. E., Veach S. R., Miller E., Dray S. Cell migration inhibition factor released by antigen from human peripheral lymphocytes. Nature. 1968 Aug 17;219(5155):755–757. doi: 10.1038/219755a0. [DOI] [PubMed] [Google Scholar]

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