Abstract
Wax D fractions obtained from young cultures (7 day) of Mycobacterium phlei and Mycobacterium smegmatis were found to contain more peptidoglycolipid, than those from old (28-day) cultures. The wax closely resembled Wax D from human strains of mycobacteria. These peptidoglycolipid fractions acted as adjuvants, producing a local granuloma at the site of injection, increasing the size of homolateral lymph nodes, inducing a delayed-type hypersensitivity (corneal response) against ovalbumin and increasing serum anti-ovalbumin precipitin levels. With extended periods of cultivation (4–6 weeks) the amount of extractable peptidoglycolipid from saprophytic organisms decreased until finally only adjuvant-inactive Wax D (glycolipid) preparations were obtained.
The hydrosoluble moiety of the peptidoglycolipid contains those amino acids, hexosamines and sugars that are found in the mucopeptide of the bacterial cell wall. It is suggested that in the absence of nutrient in the culture medium, mycobacterial growth is maintained by autonutrition from the peptidoglycolipid.
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- BOURRILLON R., MICHON J. Résolution et détermination quantitative par chromatographie sur papier des glucides liés aux protéines. Bull Soc Chim Biol (Paris) 1959;41(2-3):267–276. [PubMed] [Google Scholar]
- HOARE D. S., WORK E. The stereoisomers of alpha epsilon-diaminopimelic acid. II. Their distribution in the bacterial order Actinomycetales and in certain Eubacteriales. Biochem J. 1957 Mar;65(3):441–447. doi: 10.1042/bj0650441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOLLES P., SAMOUR D., LEDERER E. ISOLEMENT DE FRACTIONS PEPTIDO-GLYCOLIPIDIQUES 'A PARTIR DES CIRES D DE MYCOBACT'ERIES BOVINES, ATYPIQUES, AVIAIRES ET SAPROPHYTES. Biochim Biophys Acta. 1963 Oct 29;78:342–350. doi: 10.1016/0006-3002(63)91644-5. [DOI] [PubMed] [Google Scholar]
- JOLLES P., SMOUR D., LEDERER E. Analytical studies on wax D, a macromolecular peptidoglycoplied fraction from humn strains of Mycobacterium tuberculosos. Arch Biochem Biophys. 1962 Sep;Suppl 1:283–289. [PubMed] [Google Scholar]
- Jermyn M. A., Isherwood F. A. Improved separation of sugars on the paper partition chromatogram. Biochem J. 1949;44(4):402–407. doi: 10.1042/bj0440402. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEDERER E. Chemistry and biochemistry of some biologically active bacterial lipids. Pure Appl Chem. 1961;2:587–605. doi: 10.1351/pac196102030587. [DOI] [PubMed] [Google Scholar]
- RHODES M. B., AZARI P. R., FEENEY R. E. Analysis, fractionation, and purification of egg white proteins with cellulose-cation exchanger. J Biol Chem. 1958 Jan;230(1):399–408. [PubMed] [Google Scholar]
- STEWART-TULL D. E., WHITE R. G. THE OCCURRENCE OF MURAMIC ACID IN WAX D PREPARATIONS OF MYCOBACTERIA. J Gen Microbiol. 1964 Jan;34:43–49. doi: 10.1099/00221287-34-1-43. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITE R. G., BERNSTOCK L., JOHNS R. G., LEDERER E. The influence of components of M. tuberculosis and other Mycobacteria upon antibody production to ovalbumin. Immunology. 1958 Jan;1(1):54–66. [PMC free article] [PubMed] [Google Scholar]
- WHITE R. G., JOLLES P., SAMOUR D., LEDERER E. CORRELATION OF ADJUVANT ACTIVITY AND CHEMICAL STRUCTURE OF WAX D FRACTIONS OF MYCOBACTERIA. Immunology. 1964 Mar;7:158–171. [PMC free article] [PubMed] [Google Scholar]