Abstract
Human γG-globulin fractions rich in the dimeric (10S) form have been isolated from Cohn (Squibb) preparations, by repeated gel-filtration on Sephadex G-200. These fractions have been shown to fix complement and to precipitate rhemutaoid factor, despite the absence of larger aggregates (e.g. 20–40S) with which such activities are normally associated. In contrast, native human 7S γG-globulin preparations exhibited neither activity.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARANDUN S., KISTLER P., JEUNET F., ISLIKER H. Intravenous administration of human gamma-globulin. Vox Sang. 1962;7:157–174. doi: 10.1111/j.1423-0410.1962.tb03240.x. [DOI] [PubMed] [Google Scholar]
- Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
- EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ISHIZAKA T., ISHIZAKA K. Biological activities of aggregated gamma globulin. I. Skin reactive and complement-fixing properties of heat denatured gamma globulin. Proc Soc Exp Biol Med. 1959 Aug-Sep;101:845–850. doi: 10.3181/00379727-101-25116. [DOI] [PubMed] [Google Scholar]
- JAMES K., HENNEY C. S., STANWORTH D. R. STRUCTURAL CHANGES OCCURRING IN 7S GAMMA-GLOBULINS. Nature. 1964 May 9;202:563–566. doi: 10.1038/202563a0. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- SCHULTZE H. E., SCHWICK G. [On new possibilities of intravenous gamma globulin administration]. Dtsch Med Wochenschr. 1962 Aug 24;87:1643–passim. doi: 10.1055/s-0028-1113997. [DOI] [PubMed] [Google Scholar]