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. 1967 May;12(5):565–571.

The estimation of the concentration and equilibrium constant of anti-D

N C Hughes-Jones
PMCID: PMC1409131  PMID: 6067085

Abstract

A method is described for estimating the concentration and equilibrium constant of the blood group antibody, anti-D. The principle of the method is based on the fact that the reaction between anti-D and red cells is reversible and obeys the law of mass action. D-positive red cells at five different concentrations were added to aliquots of antisera containing anti-D and the reaction allowed to come into equilibrium. The amount of anti-D bound to the red cells in each case was estimated using 125I-labelled anti-γ-globulin. The results of these estimates were then analysed according to two derivations of the law of mass action. Twenty-one examples of antisera containing anti-D were examined and the estimated range of values was as follows: concentration, 0.3–7.2 μg/ml; equilibrium constant, 1.4 × 107 to 1.2 × 109 1/mole; index of heterogeneity, a = 0.6–0.9.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COSTEA N., SCHWARTZ R., CONSTANTOULAKIS M., DAMESHEK W. The use of radioactive antiglobulin for the detection of erythrocyte sensitization. Blood. 1962 Aug;20:214–232. [PubMed] [Google Scholar]
  2. DUPUY M. E., ELLIOT M., MASOUREDIS S. P. RELATIONSHIP BETWEEN RED CELL BOUND ANTIBODY AND AGGLUTINATION IN THE ANTIGLOBULIN REACTION. Vox Sang. 1964 Jan-Feb;9:40–44. doi: 10.1111/j.1423-0410.1964.tb03766.x. [DOI] [PubMed] [Google Scholar]
  3. Fujio H., Karush F. Antibody affinity. II. Effect of immunization interval on antihapten antibody in the rabbit. Biochemistry. 1966 Jun;5(6):1856–1863. doi: 10.1021/bi00870a011. [DOI] [PubMed] [Google Scholar]
  4. Mollison P. L., Hughes-Jones N. C. Clearance of Rh-positive red cells by low concentrations of Rh antibody. Immunology. 1967 Jan;12(1):63–73. [PMC free article] [PubMed] [Google Scholar]
  5. NISONOFF A., PRESSMAN D. Heterogeneity and average combining constants of antibodies from individual rabbits. J Immunol. 1958 Jun;80(6):417–428. [PubMed] [Google Scholar]
  6. Rochna E., Hughes-Jones N. C. The use of purified 125-I-labelled anti-gamma globulin in the determination of the number of D antigen sites on red cells of different phenotypes. Vox Sang. 1965 Nov-Dec;10(6):675–686. doi: 10.1111/j.1423-0410.1965.tb05179.x. [DOI] [PubMed] [Google Scholar]
  7. WELIKY N., WEETALL H. H., GILDEN R. V., CAMPBELL D. H. THE SYNTHESIS AND USE OF SOME INSOLUBLE IMMUNOLOGICALLY SPECIFIC ADSORBENTS. Immunochemistry. 1964 Oct;1:219–229. doi: 10.1016/0019-2791(64)90045-x. [DOI] [PubMed] [Google Scholar]

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