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. 1967 May;12(5):489–504.

The release of thyroglobulin from the thyroid gland into thyroid lymphatics; the identification of thyroglobulin in the thyroid lymph and in the blood of monkeys by physical and immunological methods and its estimation by radioimmunoassay

P M Daniel, O E Pratt, I M Roitt, G Torrigiani
PMCID: PMC1409139  PMID: 4960540

Abstract

The iodoprotein which was found in the lymph draining from the thyroid gland of monkeys has been identified as thyroglobulin, both by physical and by immunological techniques.

A sensitive and highly specific radioimmunoassay was developed by which thyroglobulin has been estimated in the thyroid lymph and in the blood of these animals.

Small but appreciable concentrations of thyroglobulin were found in thyroid venous and in peripheral blood. Non-thyroid lymph did not usually contain detectable concentrations of thyroglobulin but thyroglobulin was regularly found in thyroid lymph, sometimes in high concentrations. Thyroid stimulating hormone raised the concentration of thyroglobulin in the thyroid lymph still higher as did gentle massage of the tissues overlying the gland.

It was shown that the release of thyroglobulin into the thyroid lymph was a normal physiological process, for the possibility that it might have been released as a result of radiation or operative damage to the thyroid gland was excluded by experiments in which the need for administration of radioisotope to the animals was avoided and in which samples of lymph were obtained by cannulation of a cervical lymphatic trunk at some distance from the thyroid gland itself.

The implications of these findings are discussed in relation to the autoimmune phenomena seen in human thyroid disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASSEM E. S. THYROGLOBULIN IN THE SERUM OF PARTURIENT WOMEN AND NEWBORN INFANTS. Lancet. 1964 Jan 18;1(7325):139–141. doi: 10.1016/s0140-6736(64)92224-x. [DOI] [PubMed] [Google Scholar]
  2. CRAWFORD H. J., WOOD R. M., LESSOF M. H. Detection of antibodies by fluorescent-spot technique. Lancet. 1959 Dec 26;2(7113):1173–1174. doi: 10.1016/s0140-6736(59)91743-x. [DOI] [PubMed] [Google Scholar]
  3. Clutton R. F., Harington C. R., Yuill M. E. Studies in synthetic immunochemistry: Preparation and antigenic properties of thyroxyl derivatives of proteins, and physiological effects of their antisera. Biochem J. 1938 Jul;32(7):1119–1132. doi: 10.1042/bj0321119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DANIEL P. M., GALE M. M., PLASKETT L. G., PRATT O. E. Iodoprotein in the thyroid lymph of primates. Nature. 1963 Apr 27;198:392–393. doi: 10.1038/198392a0. [DOI] [PubMed] [Google Scholar]
  5. DANIEL P. M., GALE M. M., PRATT O. E. Advantages of iodine-125 for studying thyroid function in experimental animals. Nature. 1962 Dec 15;196:1065–1066. doi: 10.1038/1961065a0. [DOI] [PubMed] [Google Scholar]
  6. DANIEL P. M., GALE M. M., PRATT O. E. Radioactive iodine in the lymph leaving the thyroid gland. Q J Exp Physiol Cogn Med Sci. 1963 Apr;48:138–145. doi: 10.1113/expphysiol.1963.sp001644. [DOI] [PubMed] [Google Scholar]
  7. DANIEL P. M., GALE M. M., PRATT O. E. THE CONCENTRATION OF RADIOACTIVE IODINE IN THE THYROID LYMPH, THYROID VENOUS BLOOD AND PERIPHERAL BLOOD OF PRIMATES. J Physiol. 1963 Nov;169:330–338. doi: 10.1113/jphysiol.1963.sp007259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DECARVALHO S., LEWIS A. J., RAND H. J., UHRICK J. R. IMMUNOCHROMATOGRAPHIC PARTITION OF SOLUBLE ANTIGENS ON COLUMNS OF INSOLUBLE DIAZO-GAMMA-GLOBULINS. Nature. 1964 Oct 17;204:265–266. doi: 10.1038/204265a0. [DOI] [PubMed] [Google Scholar]
  9. DOBYNS B. M., HIRSCH E. Z. Iodinated compounds in the lymphatic pathways leading from the thyroid. J Clin Endocrinol Metab. 1956 Jan;16(1):153–155. doi: 10.1210/jcem-16-1-153. [DOI] [PubMed] [Google Scholar]
  10. Daniel P. M., Plaskett L. G., Pratt O. E. The lymphatic and venous pathways for the outflow of thyroxine, iodoprotein and inorganic iodide from the thyroid gland. J Physiol. 1967 Jan;188(1):25–44. doi: 10.1113/jphysiol.1967.sp008121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HACKETT E., BEECH M. Transient appearance of "autoimmune" antibodies during prophylactic immunization. J Immunol. 1960 Nov;85:533–538. [PubMed] [Google Scholar]
  13. HJORT T. Determination of serum thyroglobulin by a haemagglutination-inhibition test. Acta Pathol Microbiol Scand Suppl. 1962;Suppl 154:250–251. [PubMed] [Google Scholar]
  14. HJORT T., PEDERSEN G. T. Thyroid antibodies and "thyroglobulin" in the serum in pregnant and parturient women and in newborn infants. Lancet. 1962 Aug 11;2(7250):259–263. doi: 10.1016/s0140-6736(62)90170-8. [DOI] [PubMed] [Google Scholar]
  15. IRVINE W. J. THYROID AUTO-IMMUNITY AS A DISORDER OF IMMUNOLOGICAL TOLERANCE. Q J Exp Physiol Cogn Med Sci. 1964 Jul;49:324–337. doi: 10.1113/expphysiol.1964.sp001738. [DOI] [PubMed] [Google Scholar]
  16. KAPLAN M. H., SVEC K. H. IMMUNOLOGIC RELATION OF STREPTOCOCCAL AND TISSUE ANTIGENS. III. PRESENCE IN HUMAN SERA OF STREPTOCOCCAL ANTIBODY CROSS-REACTIVE WITH HEART TISSUE. ASSOCIATION WITH STREPTOCOCCAL INFECTION, RHEUMATIC FEVER, AND GLOMERULONEPHRITIS. J Exp Med. 1964 Apr 1;119:651–666. doi: 10.1084/jem.119.4.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LING N. R. The attachment of proteins to aldehyde-tanned cells. Br J Haematol. 1961 Jul;7:299–302. doi: 10.1111/j.1365-2141.1961.tb00340.x. [DOI] [PubMed] [Google Scholar]
  18. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  19. METZGER H., EDELHOCH H. Purification of anti-thyroglobin antibodies. Nature. 1962 Jan 20;193:275–276. doi: 10.1038/193275a0. [DOI] [PubMed] [Google Scholar]
  20. Perlmann P., Hammarström S., Lagercrantz R., Gustafsson B. E. Antigen from colon of germfree rats and antibodies in human ulcerative colitis. Ann N Y Acad Sci. 1965 Jun 30;124(1):377–394. doi: 10.1111/j.1749-6632.1965.tb18972.x. [DOI] [PubMed] [Google Scholar]
  21. ROITT I. M., DONIACH D. Thyroid auto-immunity. Br Med Bull. 1960 May;16:152–158. doi: 10.1093/oxfordjournals.bmb.a069816. [DOI] [PubMed] [Google Scholar]
  22. Roitt I. M., Doniach D. Autoimmunity and disease. Sci Basis Med Annu Rev. 1965:110–123. [PubMed] [Google Scholar]
  23. WEIGLE W. O. THE INDUCTION OF AUTOIMMUNITY IN RABBITS FOLLOWING INJECTION OF HETEROLOGOUS OR ALTERED HOMOLOGOUS THYROGLOBULIN. J Exp Med. 1965 Feb 1;121:289–308. doi: 10.1084/jem.121.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WEISSMANN G. LYSOSOMES, AUTOIMMUNE PHENOMENA, AND DISEASES OF CONNECTIVE TISSUE. Lancet. 1964 Dec 26;2(7374):1373–1375. doi: 10.1016/s0140-6736(64)91163-8. [DOI] [PubMed] [Google Scholar]
  25. WOLLMAN S. H., SPICER S. S., BURSTONE M. S. LOCALIZATION OF ESTERASE AND ACID PHOSPHATASE IN GRANULES AND COLLOID DROPLETS IN RAT THYROID EPITHELIUM. J Cell Biol. 1964 May;21:191–201. doi: 10.1083/jcb.21.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]

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