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. 1967 Jul;13(1):49–61.

The transmission of bovine anti-Brucella abortus agglutinins across the gut of suckling rats

I G Morris
PMCID: PMC1409144  PMID: 4961393

Abstract

The transmission across the gut of suckling rats of several types of anti-Brucella abortus agglutinins separated from a bovine immune serum by electrophoresis, gel-filtration and chromatography has been investigated.

When the immune serum was subjected to immunoelectrophoresis a minor component distinct from the main classes of immunoglobulins was detected in the γ-region. The mean electrophoretic mobility of this component was considerably lower than that of slow γG. The anti-B. abortus agglutinins of the immune serum were tentatively identified with some of the immunoglobulins because of similarities in their electrophoretic and chromatographic behaviour. Sixty to 70 per cent of the antibody activity was due to γM agglutinins. Most of the remaining activity was due to fast γG agglutinins, and less than 5 per cent was due to more slowly migrating γ-globulins. The latter could be the slow γG, the minor slow component, or both.

Chromatographic preparations of the above three types of antibodies were fed to young rats which were killed and bled 4 hours later and their sera titrated for anti-B. abortus agglutinins. The γM agglutinins were not transmitted across the gut in detectable amounts. Transmission of the fast γG agglutinins was of a very low order. The slow γG or the minor slow component agglutinins were transmitted readily and were detected in the circulation as incomplete agglutinins. It was not possible to determine whether the incomplete agglutinins appearing in the circulation of fed rats were transmitted preferentially from the preparation administered or whether complete agglutinins suffered a change into the incomplete type during transmission.

The transmission of the agglutinins is discussed in relation to their physicochemical and immunochemical properties.

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Selected References

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