Abstract
Immunogenic killed vaccine against African Horse Sickness can be prepared from a neurotropic vaccine strain or a virulent strain of virus, type 9, grown in a monkey kidney stable cell line. Virus was inactivated with either formaldehyde in a final concentration of 1:8000 or β-propiolactone at 0·2 per cent. In order to enhance the immunogenicity of the product, aluminium hydroxide was added to the vaccine as an adjuvant.
After inoculation of a single dose of either vaccine, neutralizing antibodies developed with 4 weeks, and all horses resisted challenge with homologous virulent virus. When two injections of inactivated vaccine were administered at an interval of 4 weeks, much higher neutralizing antibodies were present in sera and 6 months later all horses were still resistant to a challenge dose of virulent virus.
Under the conditions of these experiments a significantly higher degree of antigenicity was demonstrated with formalin-inactivated vaccine than with β-propiolactone-inactivated vaccine.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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