Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1968 Jan;14(1):115–126.

Mode of action of anti-lymphocyte globulin

II. Changes in the lymphoid cell population in rats treated with anti-lymphocyte globulin

A M Denman, E P Frenkel
PMCID: PMC1409258  PMID: 4169722

Abstract

Lymphopenia in rats which had been made tolerant to normal rabbit immunoglobulin G(IgG) was induced by the administration of rabbit anti-rat immunoglobulin G(IgG). The rats were injected with tritiated thymidine ([3H] thymidine) and the labelling pattern in lymphoid tissues was studied. The thymus weight decreased with continued lymphopenia and this could be explained by the release of small lymphocytes into the circulation. Lymphopoiesis in the thymus and spleen was not inhibited by anti-lymphocyte globulin. Plasmacytosis was noted in the lymph nodes. These findings support the idea that anti-lymphocyte globulin acts mainly on peripheral lymphocytes and suppresses the immune function mediated by these cells.

Full text

PDF

Images in this article

120-1FIG. 5
on p.120-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRADDOCK C. G., NAKAI G. S., FUKUTA H., VANSLAGER L. M. PROLIFERATIVE ACTIVITY OF THE LYMPHATIC TISSUES OF RATS AS STUDIED WITH TRITIUM-LABELED THYMIDINE. J Exp Med. 1964 Sep 1;120:389–412. doi: 10.1084/jem.120.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DOUGHERTY T. F. Effect of hormones on lympatic tissue. Physiol Rev. 1952 Oct;32(4):379–401. doi: 10.1152/physrev.1952.32.4.379. [DOI] [PubMed] [Google Scholar]
  3. Denman A. M., Frenkel E. P. Mode of action of anti-lymphocyte globulin. I. The distribution of rabbit anti-lymphocyte globulin injected into rats and mice. Immunology. 1968 Jan;14(1):107–113. [PMC free article] [PubMed] [Google Scholar]
  4. Ernström U., Glyllensten L., Larsson B. Venous output of lymphocytes from the thymus. Nature. 1965 Jul 31;207(996):540–541. doi: 10.1038/207540b0. [DOI] [PubMed] [Google Scholar]
  5. FRENKEL E. P., SUGINO Y., BISHOP R. C., POTTER R. L. EFFECT OF X-RADIATION ON DNA METABOLISM IN VARIOUS TISSUES OF THE RAT. VI. CORRELATIVE MORPHOLOGIC AND BIOCHEMICAL CHANGES DURING THE REGENERATION OF THE THYMUS. Radiat Res. 1963 Aug;19:701–716. [PubMed] [Google Scholar]
  6. GRASBECK R., NORDMAN C., DELACHAPELLE A. MITOGENIC ACTION OF ANTILEUCOCYTE IMMUNE SERUM ON PERIPHERAL LEUCOCYTES IN VITRO. Lancet. 1963 Aug 24;2(7304):385–386. doi: 10.1016/s0140-6736(63)93062-9. [DOI] [PubMed] [Google Scholar]
  7. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holt L. J., Ling N. R., Stanworth D. R. The effect of heterologous antisera and rheumatoid factor on the synthesis of DNA and protein by human peripheral lymphocytes. Immunochemistry. 1966 Sep;3(5):359–371. doi: 10.1016/0019-2791(66)90174-1. [DOI] [PubMed] [Google Scholar]
  9. Jeejeebhoy H. F. Immunological studies on the rat thymectomized in adult life. Immunology. 1965 Nov;9(5):417–425. [PMC free article] [PubMed] [Google Scholar]
  10. KLEIN J. J., GOLDSTEIN A. L., WHITE A. ENHANCEMENT OF IN VIVO INCORPORATION OF LABELED PRECURSORS INTO DNA AND TOTAL PROTEIN OF MOUSE LYMPH NODES AFTER ADMINISTRATION OF THYMIC EXTRACTS. Proc Natl Acad Sci U S A. 1965 Apr;53:812–817. doi: 10.1073/pnas.53.4.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOSPALLUTO J., MILLER W., Jr, DORWARD B., FINK C. W. The formation of macroglobulin antibodies. I. Studies on adult humans. J Clin Invest. 1962 Jul;41:1415–1421. doi: 10.1172/JCI104596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NOSSAL G. J. STUDIES ON THE RATE OF SEEDING OF LYMPHOCYTES FROM THE INTACT GUINEA PIG THYMUS. Ann N Y Acad Sci. 1964 Nov 30;120:171–181. doi: 10.1111/j.1749-6632.1964.tb34715.x. [DOI] [PubMed] [Google Scholar]
  13. POTTER R. L., NYGAARD O. F. The conversion of thymidine to thymine nucleotides and deoxyribonucleic acid in vivo. J Biol Chem. 1963 Jun;238:2150–2155. [PubMed] [Google Scholar]
  14. Parrott D. V., De Sousa M. A., East J. Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med. 1966 Jan 1;123(1):191–204. doi: 10.1084/jem.123.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  16. SUGINO Y., FRENKEL E. P., POTTER R. L. EFFECT OF X-RADIATION ON DNA METABOLISM IN VARIOUS TISSUES OF THE RAT. V. DNA METABOLISM IN REGENERATING THYMUS. Radiat Res. 1963 Aug;19:682–700. [PubMed] [Google Scholar]
  17. Sainte-Marie G., Leblond C. P. Elaboration of a model for the formation of lymphocytes in the thymic cortex of young adult rats. Blood. 1965 Dec;26(6):765–783. [PubMed] [Google Scholar]
  18. Turk J. L., Willoughby D. A. Central and peripheral effects of anti-lymphocyte sera. Lancet. 1967 Feb 4;1(7484):249–251. doi: 10.1016/s0140-6736(67)91307-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES