Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1968 Jan;14(1):53–59.

Fowl antibody

IX. The different responses to the hapten and carrier moieties of 2,4-dinitrophenyl-bovine γ-globulin

Eva Orlans, B Jill Saunders, M Elaine Rose
PMCID: PMC1409264  PMID: 4169725

Abstract

Fowls given one injection of 2,4-dinitrophenyl-bovine γ-globulin (DNP-BGG) intravenously or subcutaneously with complete Freund's adjuvant, or multiple injections intramuscularly, responded neither to the hapten nor to the carrier moieties of the antigen. DNP-BGG injected directly into the spleen gave rise to antibodies that precipitated with native BGG, in 0·9 per cent NaCl only, but not with the conjugated protein. Two subcutaneous injections of DNP-BGG mixed with complete adjuvant produced high levels of anti-DNP antibodies. These precipitated with DNP-ovalbumin only in 4 per cent NaCl; there were no precipitating antibodies to BGG.

Full text

PDF
53

Images in this article

56-1FIG. 1-4
on p.56-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., White R. G., Wilkinson P. C. Production of gamma-1- and gamma-2-antiovalbumin by various lymphoid tissues of the guinea pig. Immunochemistry. 1965 Dec;2(4):329–336. doi: 10.1016/0019-2791(65)90033-9. [DOI] [PubMed] [Google Scholar]
  2. BENEDICT A. A., HERSH R. T., LARSON C. THE TEMPORAL SYNTHESIS OF CHICKEN ANTIBODIES. THE EFFECT OF SALT ON THE PRECIPITIN REACTION. J Immunol. 1963 Dec;91:795–802. [PubMed] [Google Scholar]
  3. DANIEL T. M., STAVITSKY A. B. PASSIVE HEMAGGLUTINATION IN STUDY OF ANTIGENS AND ANTIBODIES. Methods Med Res. 1964;10:152–157. [PubMed] [Google Scholar]
  4. DREESMAN G., LARSON C., PINCKARD R. N., GROYON R. M., BENEDICT A. A. ANTIBODY ACTIVITY IN DIFFERENT CHICKEN GLOBULINS. Proc Soc Exp Biol Med. 1965 Jan;118:292–296. doi: 10.3181/00379727-118-29823. [DOI] [PubMed] [Google Scholar]
  5. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  6. GILDEN R. V. Antibody responses after successive injections of related antigens. Immunology. 1963 Jan;6:30–36. [PMC free article] [PubMed] [Google Scholar]
  7. GOLD E. F., BENEDICT A. A. Formation of antibody to haptens. I. Primary and secondary responses to p-amino benzoic acid and bovine gamma-globulin in chickens and rabbits. J Immunol. 1962 Aug;89:234–241. [PubMed] [Google Scholar]
  8. MARRACK J. R. SENSITIVITY AND SPECIFICITY OF METHODS OF DETECTING ANTIBODIES. Br Med Bull. 1963 Sep;19:178–182. doi: 10.1093/oxfordjournals.bmb.a070053. [DOI] [PubMed] [Google Scholar]
  9. Orlans E. Fowl antibody. 8. A comparison of natural, primary and secondary antibodies to erythrocytes in hen sera; their transmission to yolk and chick. Immunology. 1967 Jan;12(1):27–37. [PMC free article] [PubMed] [Google Scholar]
  10. Orlans E. Fowl antibody. X. The purification and properties of an antibody to the 2,4-dinitrophenyl group. Immunology. 1968 Jan;14(1):61–67. [PMC free article] [PubMed] [Google Scholar]
  11. Rosenquist G. L., Campbell G. R. Characteristics of the 19S and 7S response to bacteriophage 0X74 in the fowl. Immunology. 1966 Feb;10(2):169–178. [PMC free article] [PubMed] [Google Scholar]
  12. SZENBERG A., LIND P., CLARKE K. IGG AND IGM ANTIBODIES IN FOWL SERUM. Aust J Exp Biol Med Sci. 1965 Jul;43:451–454. doi: 10.1038/icb.1965.79. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES