Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1968 Feb;14(2):175–180.

In vitro response of the bovine peripheral lymphocyte to stimulation with cellular antigens of group B streptococcus and phytohaemagglutinin

R H Kelly
PMCID: PMC1409288  PMID: 5640943

Abstract

This investigation was conducted in two parts: first establishing separatory and cultural procedures for bovine lymphocytes, and then studying the in vitro response of these cells following stimulation with phytohaemagglutinin (PHA) and cellular constituents of Streptococcus agalactiae (St. ag.).

Peripheral lymphocytes from normal animals, those with chronic St. ag. udder infections, and animals hyperimmunized with cellular constituents of St. ag. were studied. The bovine lymphocyte responded to PHA with blastogenic changes similar to those of the human lymphocyte. No changes in cellular morphology or cytochemistry were detected following in vitro stimulation with cell antigens of St. ag. Lymphocytes from hyperimmunized animals were shown to be capable of in vitro transformation in response to a different antigenic stimulation. Several explanations for this immunological inertia are presented.

Full text

PDF
175

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle W. An antigen common to mouse cells and Salmonella typhimurium. J Immunol. 1967 Feb;98(2):256–259. [PubMed] [Google Scholar]
  2. CAMPBELL S. G., NORCROSS N. L. ANTIBODIES AGAINST STREPTOCOCCUS AGALACTIAE IN THE COLOSTRUM OF FIRST-CALF HEIFERS. Am J Vet Res. 1964 Jul;25:993–997. [PubMed] [Google Scholar]
  3. Cooper E. H., Amiel J. L. La prolifération des lymphocytes humains "in vitro". Rev Fr Etud Clin Biol. 1965 May;10(5):469–487. [PubMed] [Google Scholar]
  4. DART L. H., Jr, TURNER T. R. Fluorescence microscopy in exfoliative cytology. Report of acridine orange examination of 5491 cases, with comparison by the Papanicolaou technic. Lab Invest. 1959 Nov-Dec;8:1513–1522. [PubMed] [Google Scholar]
  5. Dodd K., Norcross N. L. Cross-reactive antigen shared by Streptococcus agalactiae and certain bovine tissues. J Bacteriol. 1967 Feb;93(2):577–579. doi: 10.1128/jb.93.2.577-579.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HALBERT S. P., BIRCHER R., DAHLE E. Studies on the mechanism of the lethal toxic action of streptolysin "O" and the protection by certain antiserotonin drugs. J Lab Clin Med. 1963 Mar;61:437–452. [PubMed] [Google Scholar]
  7. MACKINNEY A. A., Jr DOSE-RESPONSE CURVE OF PHYTOHAEMAGGLUTININ IN TISSUE CULTURE OF NORMAL HUMAN LEUCOCYTES. Nature. 1964 Dec 5;204:1002–1003. doi: 10.1038/2041002b0. [DOI] [PubMed] [Google Scholar]
  8. NORCROSS N. L. ANTIGENIC SUBSTANCES PURIFIED FROM STREPTOCOCCUS AGALACTIAE: EXTRACELLULAR PRODUCTS. Am J Vet Res. 1964 Sep;25:1457–1463. [PubMed] [Google Scholar]
  9. NORCROSS N. L. Antigenic substances purified from Streptococcus agalactiae. I. Antibody response in infected cattle. Cornell Vet. 1963 Apr;53:301–308. [PubMed] [Google Scholar]
  10. REISS E., MERTENS E., EHRICH W. E. Agglutination of bacteria by lymphoid cell in vitro. Proc Soc Exp Biol Med. 1950 Aug;74(4):732–735. doi: 10.3181/00379727-74-18030. [DOI] [PubMed] [Google Scholar]
  11. Willoughby R. A. Bovine staphylococcic mastitis: an immunohistochemical study of the cellular sites of antibody formation. Am J Vet Res. 1966 Mar;27(117):522–532. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES