Abstract
A new class of normal immunoglobulin corresponding to a myeloma protein (myeloma-IgND), which fails to react with specific antisera to IgA, D, G and M (Johansson and Bennich, 1967) was detected in serum from sixty-two blood donors using a radio-immunosorbent technique (Wide and Porath, 1966).
IgND in normal sera corresponds to myeloma-IgND in Ouchterlony gel diffusion analysis.
Isolated IgND gave a reaction of identity with myeloma-IgND in Ouchterlony gel diffusion analysis.
The concentrations of IgND in 93·5 per cent of the samples was within the range of 100–700 ng/ml.
Normal levels of IgND were found in four samples apparently lacking IgA and/or IgD as determined by single radial immunodiffusion.
Elevated levels of IgND were found in four samples one of which was from a subject with previously undiagnosed extrinsic asthma.
It is concluded that myeloma-IgND represents a new class of human immunoglobulin.
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- Axén R., Porath J. Chemical coupling of enzymes to cross-linked dextran ('Sephadex'). Nature. 1966 Apr 23;210(5034):367–369. doi: 10.1038/210367a0. [DOI] [PubMed] [Google Scholar]
- EGGSTEIN M., KREUTZ F. H. Vergleichende Untersuchungen zur quantitativen Eiweissbestimmung im Liquor und eiweissarmen Lösungen. Klin Wochenschr. 1955 Oct 1;33(37-38):879–884. doi: 10.1007/BF01473099. [DOI] [PubMed] [Google Scholar]
- HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
- Johansson S. G., Bennich H. Immunological studies of an atypical (myeloma) immunoglobulin. Immunology. 1967 Oct;13(4):381–394. [PMC free article] [PubMed] [Google Scholar]
- KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN. J Exp Med. 1965 Jan 1;121:171–184. doi: 10.1084/jem.121.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]